The effect of inhibitors of photosynthesis on zooxanthellae in corals and other marine invertebrates
The effect of inhibitors of photosynthesis on zooxanthellae in corals and other marine invertebrates
复制标题
光合作用抑制剂对珊瑚和其他海洋无脊椎动物中虫黄藻的影响
DOI：
10.1007/bf00346940
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发表时间：
1972
期刊：
影响因子：
2.4
通讯作者：
L. Muscatine
中科院分区：
文献类型：
--
作者：
J. Vandermeulen;N. Davis;L. Muscatine
研究了选择性光合作用抑制剂灭草隆（CMU）、敌草隆（DCMU）和甲基紫精对藻类-海洋无脊椎动物共生关系的影响。CMU或DCMU（5x 10 - 4 M）完全抑制光合作用，无论是在完整的分支，并在悬浮液中的孤立zooxanthropium从造礁珊瑚Pocillopora damicornis。抑制作用在去除抑制剂后1 ~ 3 h内完全可逆。类似的抑制光合作用发生在8个其他海洋腔肠动物共生与zooxanthemum，并在1海洋腹足类共生功能叶绿体。CMU和DCMU均不影响宿主的游泳、趋光性、向光性、光感受、触角收缩、纤毛跳动和运动等行为。甲基紫精在低浓度下对完整的棉角萤叶甲的光合作用没有抑制作用，而在高浓度下对棉角萤叶甲的组织有致死作用。这些观察表明，CMU和DCMU是潜在的有用的工具，调查共生协会。DCMU（5 × 10 - 4 M）也可逆地抑制了光增强的钙化。这有力地表明，光增强钙化在很大程度上依赖于光合作用，而可能不依赖于其他一些光生物学效应。
The effect of the selective photosynthesis inhibitors Monuron (CMU), Diuron (DCMU) and methyl viologen on intact algal-marine invertebrate symbiotic associations was studied. CMU or DCMU (5x10-4M) completely inhibited photosynthesis, both in intact branches, and in suspensions of isolated zooxanthellae from the reef-building coral Pocillopora damicornis. The inhibitory effect was totally reversible in 1 to 3 h after removal of the inhibitor. Similar inhibition of photosynthesis occurred in 8 other marine coelenterates symbiotic with zooxanthellae, and in 1 marine gastropod symbiotic with functional chloroplasts. Neither CMU nor DCMU appeared to affect behavior of the various hosts, such as swimming, phototaxis, phototropism, photoreception, tentacle contraction, ciliary beating and locomotion. Methyl viologen, however, was ineffective in inhibiting photosynthesis in intact P. damicornis at low concentrations, and lethal to the tissues at high concentrations. These observations indicate that CMU and DCMU are potential useful tools for investigation of symbiotic associations. DCMU (5x10-4M) also reversibly inhibited light-enhanced calcification in P. damicornis. This strongly suggests that light-enhanced calcification is largely photosynthesis dependent, and probably not dependent on some other photobiological effect.