Microwave Treatment Prevents Cytomegalovirus Transmission to Preterm Infants through Human Milk
Microwave Treatment Prevents Cytomegalovirus Transmission to Preterm Infants through Human Milk
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微波治疗可防止巨细胞病毒通过母乳传播给早产儿
DOI：
10.26502/jfsnr.2642-110000103
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Den H
中科院分区：
文献类型：
--
作者：
Mizuno K;Kohda C;Den H
背景资料：母乳是早产儿的最佳营养;然而，巨细胞病毒（CMV）血清阳性母亲的乳汁可能会将病毒传播给婴儿。由于极早产儿由于CMV感染而有发展严重疾病的风险，即使是婴儿有时也会进行巴氏消毒。以前，我们证明，微波加热50毫升配方奶含有CMV在500 W的40秒，防止CMV感染活细胞。然而，我们不知道这是否是人乳（HM）中再活化CMV的情况。因此，我们研究了微波加热HM是否能有效预防活细胞中的CMV感染。研究方法：23个CMV-IgG阳性和4个CMV-IgG阴性的母亲谁捐赠HM的母乳银行在产后6至16周内入组。用500 W的微波处理HM。将HFL-III细胞接种在培养皿中。将经过微波处理的HM样品的等分试样接种到易感细胞单层上。通过50%中位组织培养感染剂量（TCID 50）试验测量感染性小瓶滴度。结果：100 mL样品经200 W微波处理60 s后，5/8样品的TCID 50降低。CMV感染性试验在3份100 mL样本中为阴性，8份降低，6份无变化，4份50 mL样本中为阴性，8份降低。结论：在低设置下微波加热HM可以减少CMV通过HM的传播，然而，当考虑到生物活性特性的损失时，很难获得完全的预防。
Background: Mother’s own milk (MOM) is the best nutrition for preterm infants; however, milk from cytomegalovirus (CMV)-seropositive mothers may cause the transmission of the virus to the infants. As extremely preterm infants are at a risk of developing serious conditions due to CMV infection, even MOM is sometimes pasteurized. Previously, we demonstrated that microwave heating of 50 mL formula milk containing CMV at 500 W for 40s prevented CMV infection in living cells. However, we do not know if this is the case with reactivated CMV in human milk (HM). Therefore, we investigated if microwave heating of HM would be effective in preventing CMV infection in living cells. Methods: Twenty-three CMV-IgG-positive and four CMV-IgG-negative mothers who donated HM to the human milk bank during 6 to 16 weeks postpartum were enrolled. The HM was treated with microwave at 500 W. HFL-III cells were seeded in culture dishes. Aliquots of HM samples subjected to microwave was inoculated onto susceptible cell monolayers. Infectious vial titer was measured by the 50% median tissue culture infectious Dose (TCID50) assay. Results: The TCID50 assay was decreased in five out of eight 100-mL samples after microwave at 200W for 60 s. The CMV infectivity assay was negative in three, decreased in eight, and no change in six 100-mL samples, and negative in four and decreased in eight 50-mL samples. Conclusions: Microwave heating of HM at low settings could decrease CMV transmission through HM, however, to obtain entire prevention is difficult when loss of bioactive properties is considered.
影响因子：
2.6
作者：
Yoshida Y;Azuma M;Furukawa K;Mizuno K;Den H;Kamiya T;Izumizaki M
通讯作者：
Izumizaki M