OsPNH1 regulates leaf development and maintenance of the shoot apical meristem in rice
OsPNH1 regulates leaf development and maintenance of the shoot apical meristem in rice
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DOI：
10.1046/j.1365-313x.2002.01279.x
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发表时间：
2002-04-01
期刊：
影响因子：
7.2
通讯作者：
Matsuoka, M
中科院分区：
文献类型：
--
作者：
Nishimura, A;Ito, M;Matsuoka, M
拟南芥 PINHEAD/ZWILLE (PNH/ZLL) 基因被认为在茎尖分生组织 (SAM) 的形成和叶近轴细胞规格中发挥重要作用。为了研究水稻发育的分子机制，我们分离了 PNH/ZLL 的水稻同源物，称为 OsPNH1。在 SAM 周围，OsPNH1 在发育中的叶原基中强烈表达，特别是在假定的维管结构域、发育中的维管组织、近轴区域的一些细胞层以及未来的束鞘延伸细胞中。在 SAM 中，仅在中心区域观察到弱表达，而在外围 SAM 域的叶创始细胞的中脉区域检测到强表达。我们培育出含有反义 OsPNH1 链的转基因水稻植物。反义 OsPNH1 植物发育出畸形的叶子，具有改变的维管束排列和异常的内部结构。这些植物还形成了 KNOX 基因表达降低的异常 SAM。我们检查了 OsPNH1-GFP 融合蛋白的亚细胞定位，发现它位于细胞质中。基于这些观察，我们提出 OsPNH1 不仅在 SAM 维持中发挥作用（如先前认为的那样），而且还在通过维管发育形成叶子中发挥作用。
The Arabidopsis PINHEAD/ZWILLE (PNH/ZLL) gene is thought to play an important role in the formation of the shoot apical meristem (SAM) and in leaf adaxial cell specification. To investigate the molecular mechanisms of rice development, we have isolated a rice homologue of PNH/ZLL , called OsPNH1. Around the SAM, OsPNH1 was strongly expressed in developing leaf primordia, specifically in the presumptive vascular domains, developing vascular tissues, a few cell-layers of the adaxial region, and future bundle sheath extension cells. In the SAM, only weak expression was observed in the central region, whereas strong expression was detected in the mid-vein region of leaf founder cells in the peripheral SAM domain. We produced transgenic rice plants containing the antisense OsPNH1 strand. The antisense OsPNH1 plants developed malformed leaves with an altered vascular arrangement and abnormal internal structure. These plants also formed an aberrant SAM with reduced KNOX gene expression. We examined the subcellular localization of the OsPNH1-GFP fusion protein and found that it was localized in the cytoplasm. On the basis of these observations, we propose that OsPNH1 functions not only in SAM maintenance as previously thought, but also in leaf formation through vascular development.