Use of capillary electrophoresis as a method development tool for classical gel electrophoresis.
Use of capillary electrophoresis as a method development tool for classical gel electrophoresis.
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使用毛细管电泳作为经典凝胶电泳的方法开发工具。
DOI：
10.1039/a801340c
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Stalcup,AM
中科院分区：
文献类型：
--
作者：
Sutton,RM;Gratz,SR;Stalcup,AM
采用毛细管电泳法(CE)优化了缓冲溶液pH、离子强度和环糊精浓度对哌罗生对映体拆分的影响。然后将这些对映体分离条件应用于经典的凝胶电泳法。用毛细管电泳法计算了磺化β-环糊精-哌咯烷偶对的结合常数，并考察了有机溶剂对体系的影响。
Capillary electrophoresis (CE) was used to optimize the buffer pH, ionic strength and sulfated cyclodextrin concentrations for enantiomeric separation of piperoxan. These enantioseparation conditions were then applied to a classical gel electrophoresis system. Binding constants of the sulfated β-cyclodextrin–piperoxan couple were approximated using CE and the effects of organic solvents on the system were also investigated.