A rapid method for the measurement of sex hormone binding globulin capacity of sera.
A rapid method for the measurement of sex hormone binding globulin capacity of sera.
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一种快速测定血清性激素结合球蛋白能力的方法
DOI：
10.1016/0009-8981(74)90293-9
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
D. London
中科院分区：
文献类型：
--
作者：
B. Rudd;N. Duignan;D. London
本文介绍了一种简便、快速的测定血清性激素结合球蛋白（SHBG）的方法。[4- 14 C]替吉奥在一个剂量水平下用作除妊娠血清外所有样本的饱和配体。用硫酸铵将白蛋白结合的[4- 14 C]睾酮与球蛋白分离，并对标记球蛋白部分进行计数。在确定的最佳条件下，用活性炭混悬液预处理血清可去除与结合蛋白相关的内源性类固醇，而不影响[4- 14 C]睾酮与SHBG的结合。该方法具有良好的重现性，男性和女性血清的变异系数分别为4%和4.7%。正常男性、女性和妊娠血清在20°时的结合常数分别为3.6 × 108 M −1、3.2 × 108 M −1和1.7 × 108 M − 1。正常男性的SHBG容量为5.0 ± 0.36 × 10− 8 M/1;女性为7.80 ± 1.24;青春期前儿童为7.01 ± 1.8。闭经妇女和多囊卵巢多毛妇女的SHBG容量最低，分别为4.19 ± 0.75和4.06 ± 0.97。绝经后妇女的水平也较低，为5.25 ± 1.25。5例选择性低促性腺激素性性腺功能减退症男性的平均值为5.69 ± 0.84，明显高于正常男性。两名Kallman综合征患者的SHBG能力升高。与正常男性相比，两名全垂体功能减退症患者和一名Klinefelter综合征患者的SHBG能力较低。1例生长激素缺乏症患者的SHBG能力正常。妊娠血清的容量最高，为29.03 ± 3.76。在10名正常男性的血清中，在3天的时间内，SHBG能力没有每天的变化。
A simple rapid method for the measurement of sex hormone binding globulin (SHBG) capacity, using 0.2ml of serum, is described. [4-14C] Testosterone is used as the saturating ligand at one dose level for all samples except pregnancy sera. Albumin bound [4-14C] testosterone is separated from the globulins with ammonium sulphate and the labelled globulin fraction counted. Pre-treatment of sera with a charcoal suspension under defined optimum conditions removes endogenous steroids associated with the binding proteins without affecting the binding [4-14C] testosterone to SHBG. The method shows good reproducibility with a coefficient of variation of 4% for male and 4.7% for female serum. Association constants measured for normal male, female and pregnancy sera were 3.6 × 108M−1, 3.2 × 108M−1, and 1.7 × 108M−1at 20°, respectively.Normal males gave SHBG capacities of 5.0 ± 0.36 × 10−8M/1; females, 7.80 ± 1.24; prepubertal children, 7.01 ± 1.8. Women with amenorrhoea and hirsute women with polycystic ovaries had the lowest SHBG capacities of all, at 4.19 ± 0.75 and 4.06 ± 0.97 respectively. Post-menopausal women also had low levels, 5.25 ± 1.25. Five males with selective hypogonadotrophic hypogonadism had a mean value of 5.69 ± 0.84 which was significantly higher than the normal males. Two patients with Kallman's syndrome had elevated SHBG capacities. Two patients with panhypopituitarism and one patient with Klinefelter's syndrome had low SHBG capacities when compared with normal males. One patient with growth hormone deficiency had a normal SHBG capacity. Pregnancy sera had the highest capacities at 29.03 ± 3.76. No day to day variation in SHBG capacity could be demonstrated over a three day period in the sera from ten normal males.