JUVENILE-HORMONE AND JUVENILE-HORMONE ESTERASE IN ADULT FEMALES OF THE MOSQUITO AEDES-AEGYPTI
JUVENILE-HORMONE AND JUVENILE-HORMONE ESTERASE IN ADULT FEMALES OF THE MOSQUITO AEDES-AEGYPTI
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DOI：
10.1016/0022-1910(86)90102-2
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发表时间：
1986-01-01
影响因子：
2.2
通讯作者：
SCHOOLEY, DA
中科院分区：
文献类型：
--
作者：
SHAPIRO, AB;WHEELOCK, GD;SCHOOLEY, DA
保幼激素III水平和保幼激素酯酶活性测定全身提取物和血淋巴，分别为女性埃及伊蚊。保幼激素的量，通过耦合气相色谱-质谱法测定，上升后的第一个2天出现从0.7到7.5纳克/克，然后在接下来的5天内缓慢下降，在女性没有提供血餐。在喂食血液的雌性中，保幼激素水平在前3小时内下降至2.3纳克/克。然后下降速度减慢，在血餐后24小时达到最低点（0.4纳克/克）。到48小时，水平再次开始上升，直到96小时，当它们相当于血餐前的水平。保幼激素酯酶活性的女性血淋巴中测定与分配试验。酯酶活性在未进食的动物中表现出小的波动。羽化后第3天吸血的雌虫，保幼激素酯酶活性缓慢上升，36 h达高峰。在42小时，它开始下降，到66小时，它已恢复到血餐前的水平。因此，保幼激素水平和保幼激素酯酶活性呈负相关后，血餐。在器官培养中，卵巢和脂肪体都产生保幼激素酯酶。保幼激素III酸是唯一的代谢产物与外消旋标记保幼激素III孵育后产生的血淋巴。保幼激素酸，二醇，酸二醇的主要代谢产物，在整个动物提取物后局部应用标记的激素。大约25%的局部应用的标记保幼激素以酸性二醇的形式出现在血淋巴中，其中50%在血餐后立即通过尿液排泄。局部应用BEPAT（S-苄基-O-乙基磷酰胺硫醇盐），保幼激素酯酶的特异性抑制剂，导致保幼激素酸的缺乏和酸二醇的减少。BEPAT和methoprene，保幼激素类似物，造成减少卵孵化时，局部应用30小时后的血餐，表明血餐后保幼激素水平的下降是必要的正常卵发育，并表明下降介导的，至少部分，由保幼激素酯酶。
Juvenile hormone III levels and juvenile hormone esterase activity were measured in whole body extracts and haemolymph, respectively, of female Aedes aegypti. The amount of juvenile hormone, determined by coupled gas chromatography-mass spectrometry, rose over the first 2 days after emergence from 0.7 to 7.5 ng/g, and then slowly fell over the next 5 days in females not given a blood meal. In females fed blood, juvenile hormone levels fell during the first 3 h to 2.3 ng/g. The rate of decline then slowed so that levels had reached their lowest point (0.4 ng/g) by 24 h after the blood meal. By 48 h, levels started to rise again until 96 h when they were equivalent to pre-blood meal levels. Juvenile hormone esterase activity in the haemolymph of females was measured with a partition assay. The esterase activity showed small fluctuations in unfed animals. In females fed blood on the 3rd day after emergence, the juvenile hormone esterase activity rose slowly to a peak at 36 h. At 42 h it began to decline, and by 66 h it had returned to pre-blood meal levels. Thus, juvenile hormone levels and juvenile hormone esterase activity were inversely correlated after a blood meal. Both the ovary and fat body produce juvenile hormone esterase in organ culture. Juvenile hormone III acid was the only metabolite produced after incubation of haemolymph with racemic-labelled juvenile hormone III. Juvenile hormone acid, diol, and acid diol were the main metabolic products seen in whole animal extracts after topical application of labelled hormone. About 25% of topically applied, labelled juvenile hormone appears in the haemolymph as the acid diol, and 50% of this is excreted in the urine immediately after the blood meal. Topical application of BEPAT (S-benzyl-O-ethyl phosphoramidothiolate), a specific inhibitor of juvenile hormone esterase, resulted in the absence of juvenile hormone acid and a reduction in the acid diol. Both BEPAT and methoprene, a juvenile hormone analogue, caused a reduction in egg hatch when applied topically 30 h after a blood meal, demonstrating that the decline in juvenile hormone levels after a blood meal is necessary for normal egg development and suggesting that the decline is mediated, at least in part, by juvenile hormone esterase.