Diagnostic value of SOX-10 immunohistochemical staining for the detection of uveal melanoma.
Diagnostic value of SOX-10 immunohistochemical staining for the detection of uveal melanoma.
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DOI：
10.3332/ecancer.2015.566
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发表时间：
2015
影响因子：
1.8
通讯作者：
Burnier MN Jr
中科院分区：
文献类型：
--
作者：
Alghamdi SA;Zoroquiain P;Dias AB;Alhumaid SR;Aldrees S;Burnier MN Jr
SOX-10 已被证明是皮肤黑色素瘤的敏感标志物。本研究旨在评估葡萄膜黑色素瘤中 Sox-10 的表达。总共切下 40 个患有葡萄膜黑色素瘤的摘除眼组织块，并使用抗 SOX-10 小鼠单克隆抗体和 HMB-45 抗体进行染色。 SOX-10 在 100% 的葡萄膜黑色素瘤病例中显示出唯一的核阳性 (38/38)。 HMB-45 显示细胞质阳性率为 97.3 (37/38)。 78% 的摘除眼睛的视网膜内核层和外核层也显示出 SOX-10 阳性。 SOX-10 表达被证明是葡萄膜黑色素瘤最敏感的标志物，因此，我们提出了一种用于诊断葡萄膜黑色素瘤的改良组合，其中包括 SOX-10 和 HMB-45。在视网膜内核层和外核层中观察到明显的弥漫性核 SOX-10 表达这一发现值得进一步研究作为视网膜母细胞瘤的标志物。
SOX-10 has been shown to be a sensitive marker of cutaneous melanoma. This study aimed to evaluate Sox-10 expression in uveal melanoma. A total of 40 tissue blocks of enucleated eyes with uveal melanoma were cut and stained using an anti-SOX-10 mouse monoclonal antibody and HMB-45 antibody. SOX-10 showed exclusive nuclear positivity in 100% of the uveal melanoma cases (38/38). HMB-45 showed cytoplasmic positivity in 97.3 (37/38). Positivity for SOX-10 was also noted in the inner and outer nuclear layers of the retina in 78% of the enucleated eyes. SOX-10 expression proved to be the most sensitive marker for uveal melanoma, and therefore, we propose a modified panel for the diagnosis of uveal melanoma that includes both SOX-10 and HMB-45. The observation of distinct, diffuse nuclear SOX-10 expression in retinal inner and outer nuclear layers is a finding that warrants further investigation as a marker for retinoblastoma.