A murine femoral segmental defect model for bone tissue engineering using a novel rigid internal fixation system
A murine femoral segmental defect model for bone tissue engineering using a novel rigid internal fixation system
复制标题
使用新型刚性内固定系统的骨组织工程鼠股骨节段缺损模型
DOI：
10.1016/j.jss.2013.02.041
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发表时间：
2013-08-01
影响因子：
2.2
通讯作者：
Li, Zubing
中科院分区：
文献类型：
--
作者：
Liu, Kai;Li, Dianqi;Li, Zubing
背景：小鼠作为一种模型动物，已被广泛应用于骨相关研究。然而，目前尚缺乏理想的长骨节段性缺损小鼠模型。由于外固定架具有重量大、穿透皮肤、影响活动能力等缺点，因此内固定装置可能更适合于维持节段性骨缺损。本研究的目的是通过设计内固定系统，验证其适应性，研究股骨节段性缺损的临界大小，建立一种简单、可重复性、标准化的小鼠临界大小缺损区模型。方法：利用计算机辅助测量和处理系统，获取成年C57BL/6小鼠股骨的解剖数据，并设计一种用于刚性固定的钢板-螺栓系统。67只小鼠固定钢板和螺钉，在股骨中段形成1.5 mm或2.0 mm的缺损区。分别于术后0、2、5、12周对小鼠骨-种植体结构进行压缩和三点弯曲测试，以测试其生物力学稳定性。采用X射线、计算机断层扫描和组织学方法观察小鼠股骨的愈合过程。结果：小鼠股骨内固定和单侧或双侧手术对小鼠总体无不良影响。力学测试表明，植入骨与完整股骨在压缩和三点弯曲载荷方面没有显著差异。微电脑断层扫描显示，固定装置的植入对骨密度无影响。结论：使用专门设计的坚固内固定装置，C57BL/6小鼠股骨2.0 mm的节段性缺损区将出现骨不连，可作为骨组织工程和骨再生研究的关键缺损区。Crown版权所有(C)2013由Elsevier Inc.出版。保留所有权利。
Background: As a model animal, the mouse has already been widely used in bone-related research. However, there is a lack of ideal long bone segmental defect mouse model. Since external fixation has disadvantages of heavy weight, penetrating the skin, and hampering mobility, an internal fixation device is probably more preferable to maintain the segmental bone defect. The aim of this study was to establish a simple, reproducible, and standardized murine critical-size defect model through designing an internal fixation system, verifying its adaptability, and investigating the critical size of femoral segmental defect.Methods: By utilizing computer-aided measuring and processing system, anatomical data of adult C57BL/6 mouse femur was obtained, and a plate-bolts system was designed for rigid fixation. The plate and screws were fixed in 67 mice and 1.5 or 2.0 mm defect gaps were created in the femoral midshaft. Compression and three-point bending of bone-implant construct were tested in mice at 0, 2, 5, and 12 wk postoperative to test the biomechanical stability. X-ray, micro-computed tomography, and histology were used to investigate the defect healing process.Results: The plate- and screws-fitted mouse femur and unilateral or bilateral operation had seemingly no adverse impact on the mouse in general. Mechanical tests indicated that there were no significant differences between the bone-implant construct and intact femur in compression and three-point bending loading. Micro-computed tomography scanning showed the bone mineral density had not been affected by the implantation of fixation device. There was no union of the 2.0 mm segmental defect in 12-wk period.Conclusion: Using the specifically designed rigid internal fixation device, a segmental defect size of 2.0 mm in C57BL/6 mouse femur will show nonunion and can serve as a critical defect size for bone tissue engineering and bone regeneration research. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved.