Cloning and functional characterization of thioredoxin gene from kuruma shrimp Marsupenaeus japonicus
Cloning and functional characterization of thioredoxin gene from kuruma shrimp Marsupenaeus japonicus
复制标题
日本库鲁玛虾硫氧还蛋白基因的克隆及功能鉴定
DOI：
10.1016/j.fsi.2018.11.064
复制
发表时间：
2019-03-01
影响因子：
4.7
通讯作者：
Kang, Cui-jie
中科院分区：
文献类型：
--
作者：
Guo, Ning-ning;Sun, Xue-jun;Kang, Cui-jie
硫氧还蛋白（Thioredoxin，Trx）作为细胞内抗氧化系统中重要的二硫还原酶，在维持氧化应激平衡和保护细胞免受氧化损伤中发挥重要作用。近年来，越来越多的证据表明Trx是多种疾病发病机制中的关键分子，是肺癌、结肠癌、宫颈癌、胃癌和胰腺癌等重大疾病的潜在治疗靶点。然而，关于Trx在病毒感染中的功能知之甚少。本研究克隆了感染白色斑点综合征病毒（WSSV）的日本囊对虾（Marsupenaeus）的Trx同源基因MjTrx，并对其功能进行了初步研究。MjTrx是一种105个氨基酸的多肽，在催化中心具有保守的Cys-Gly-Pro-Cys基序。系统进化树分析表明，MjTrx与其他无脊椎动物的Trx亲缘关系较近，与节肢动物的Trx 1聚在一起。MjTrx转录本在鳃和肠组织中丰富，并且可以在血细胞、心脏、胃和肝胰腺组织中检测到。感染白色综合征病毒后，对虾血细胞、鳃和肠组织中MjTrx的转录水平显著上调。MjTrx在体外重组表达，具有明显的二硫键还原酶活性。此外，过量表达MjTrx导致体内过氧化氢（H2O2）浓度增加。这些结果表明，MjTrx具有氧化还原稳态调节功能，在对虾抗病毒免疫中起重要作用。
As an important disulfide reductase of the intracellular antioxidant system, Thioredoxin (Trx) plays an important role in maintaining oxidative stress balance and protecting cells from oxidative damage. In recent years, there is increasing evidence that Trx is a key molecule in the pathogenesis of various diseases and a potential therapeutic target for major diseases including lung, colon, cervical, gastric and pancreatic cancer. However, few knowledge is known about the function of Trx in virus infection. In this study, we reported the cloning and functional investigation of a Trx homologue gene, named MjTrx, in shrimp Marsupenaeus japonicus suffered white spot syndrome virus (WSSV) infection. MjTrx is a 105-amino acid polypeptide with a conservative Cys-Gly-Pro-Cys motif in the catalytic center. Phylogenetic trees analysis showed that MjTrx has a higher relationship with Trx from other invertebrate and clustered with Trx1 from arthropod. MjTrx transcripts is abundant in the gill and intestine tissues and can be detected in the hemocytes, heart, stomach, and hepatopancreas tissues. The transcription levels of MjTrx in hemocytes, gills and intestine tissues of shrimp were significantly up-regulated after white spot syndrome virus infection. MjTrx was recombinant expressed in vitro and exhibited obvious disulfide reductase activity. In addition, overexpression MjTrx in shrimp resulted in the increase of hydrogen peroxide (H2O2) concentration in vivo. All these results strongly suggested that MjTrx functioned in redox homeostasis regulating and played an important role in shrimp antiviral immunity.