Identification of scuticociliate Philasterides dicentrarchi from indo-pacific seahorses Hippocampus kuda
Identification of scuticociliate Philasterides dicentrarchi from indo-pacific seahorses Hippocampus kuda
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印度太平洋海马 Hippocampus kuda 中盾纤毛虫 Philasterides dicentrarchi 的鉴定
DOI：
10.5897/ajmr10.294
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发表时间：
2011
影响因子：
--
通讯作者：
Se Chang Park
中科院分区：
文献类型：
--
作者：
S. Shin;J. Han;D. K. Gomez;Ji Hyung Kim;C. Choresca;J. Jun;Se Chang Park
在一间私人商业水族馆饲养用于展览的“印度太平洋海马”Hippocampus kuda被送往实验室进行诊断。对 5 只海马的粪便和肠道内容物进行显微镜检查，发现大量盾纤毛虫。我们通过光（湿涂片）和扫描电子显微镜、PCR（聚合酶链式反应）和测序鉴定了盾纤毛虫的种类。本文报道了韩国首次从海马中鉴定出盾纤毛虫 Philasterides dicentrarchi。
关键词：印太海马、Philasterides dicentrarchi、扫描电子显微镜、聚合酶链式反应、测序。
The “indo-pacific seahorses”, Hippocampus kuda which had been reared in one of the private commercial aquaria for exhibition were sent to the laboratory for diagnosis. Feces and intestinal contents from 5 seahorses were examined microscopically and revealed numerous scuticociliates. We identified the species of scuticociliate by light (wet mount) and scanning electron microscopy, PCR (polymerase chain reaction) and sequencing. This paper reports the first identification of scuticociliate Philasterides dicentrarchi from seahorse in Korea.
Key words: Indo-pacific seahorse, Philasterides dicentrarchi, scanning electron microscopy, polymerase chain reaction, sequencing.