Substantive molecular and histological changes within the meniscus with tears
Substantive molecular and histological changes within the meniscus with tears
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半月板内发生实质性分子和组织学变化并伴有泪液
DOI：
10.1186/s12891-019-2943-z
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发表时间：
2019-12-01
影响因子：
2.3
通讯作者：
He, Aishan
中科院分区：
文献类型：
--
作者：
Long, Yi;Xie, Jingping;He, Aishan
背景半月板在膝关节的正常生物力学中起着至关重要的作用。然而，在分子水平上对它的研究还不够深入。方法收集正常半月板6例(A组)，单纯半月板撕裂7例(B组)，半月板撕裂合并前交叉韧带撕裂7例(C组)。采用实时定量聚合酶链式反应结合免疫组织化学和原位杂交技术分别检测ACAN、ADAMTS5、COL10A1、CEBPβ、MMP13和miR-381-3p、miR-455-3p、miR-193B-3p、miR-92a-3p的表达。结果与A组比较，B组和C组膝关节组织中ADAMTS5、COL10A1、CEBPβ和MMP13的表达水平均升高，而ACAN的表达水平均下调，C组除ACAN基因表达和miRNA水平低于B组外，其余均高于B组。B组和C组多个纤维软骨细胞表达ADAMTS5、CEBPβ和MMP13，且miR381-3PandmiR455-3pP水平高于A组，B组术后Lysholm和IKDC评分高于C组。结论半月板损伤后有退变的趋势，尤其是合并前交叉韧带撕裂时。这项研究中研究的miRNAs也可能与半月板退变有关。合并伤类型患者的关节功能可能相对较差。
BackgroundThe meniscus plays a vital role in the normal biomechanics of the knee. However, it is not well studied at the molecular level. The purpose of this study was to determine whether molecular and pathological changes in the meniscal tissue vary depending on the presence or absence of meniscal and/or anterior cruciate ligament tear (ACL).MethodsSix normal menisci (group A), seven simple torn menisci (group B) and seven torn menisci with concomitant anterior cruciate ligament tears (group C) were collected. We observed the pathological changes in the menisci and used real-time polymerase chain reaction along with immunohistochemistry and in situ hybridisation to examine the levels ofACAN, ADAMTS5, COL10A1, CEBPβ, MMP13andmiR-381-3p, miR-455-3p, miR-193b-3p, miR-92a-3p,respectively. Patients were scored preoperatively and postoperatively using the Lysholm Knee Scoring Scale and International Knee Documentation Committee Subjective Knee Evaluation Form.ResultsCompared with group A, the expression levels ofADAMTS5, COL10A1, CEBPβ,andMMP13and all the miRNAs were increased whileACANwas down-regulated in groups B and C. Additionally, the gene expression and miRNA levels were higher in group C than that in group B, except forACAN, which was lower. Several fibrochondrocytes strongly expressed ADAMTS5, CEBPβ, and MMP13 in groups B and C and had high levels ofmiR-381-3pandmiR-455-3pthan that in group A. Postoperative Lysholm and IKDC scores were higher in group B than in group C.ConclusionsOur findings suggest that the meniscus tended to degenerate after it was injured, especially when combined with a torn ACL. The miRNAs investigated in this study might also contribute to meniscus degeneration. Patients with a combined injury patterns might have relatively worse joint function.