Proteoform Analysis and Construction of Proteoform Families in Proteoform Suite.
Proteoform Analysis and Construction of Proteoform Families in Proteoform Suite.
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DOI：
10.1007/978-1-0716-2325-1_7
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发表时间：
2022
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Proteoform Suite是一个交互式软件程序，用于从质谱数据中识别和定量完整的蛋白质。Proteoform Suite可识别通过完整质量（MS 1）分析观察到的蛋白质型。在完整质量分析中，将未片段化的实验蛋白质型与已知蛋白质型序列的数据库进行比较，并相互比较，搜索对应于众所周知的翻译后修饰或氨基酸的质量差异。完整质量分析能够识别在MS 1数据中观察到的蛋白质型，而无需MS/MS（MS 2）碎片化。Proteoform Suite进一步促进了蛋白质家族的构建和可视化，这些家族是源自单个基因的蛋白质组。自下而上的肽鉴定和自上而下的（MS 2）蛋白质型鉴定可以集成到Proteoform Suite分析中，以提高分析的灵敏度和准确性。Proteoform Suite是开源的，可在https://github.com/smith-chem-wisc/proteoform-suite上免费获得。
Proteoform Suite is an interactive software program for the identification and quantification of intact proteoforms from mass spectrometry data. Proteoform Suite identifies proteoforms observed by intact-mass (MS1) analysis. In intact-mass analysis, unfragmented experimental proteoforms are compared to a database of known proteoform sequences and to one another, searching for mass differences corresponding to well-known post-translational modifications or amino acids. Intact-mass analysis enables proteoforms observed in the MS1 data without MS/MS (MS2) fragmentation to be identified. Proteoform Suite further facilitates the construction and visualization of proteoform families, which are the sets of proteoforms derived from individual genes. Bottom-up peptide identifications and top-down (MS2) proteoform identifications can be integrated into the Proteoform Suite analysis to increase the sensitivity and accuracy of the analysis. Proteoform Suite is open source and freely available at https://github.com/smith-chem-wisc/proteoform-suite.