Jupiter, a new Drosophila protein associated with microtubules
Jupiter, a new Drosophila protein associated with microtubules
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DOI：
10.1002/cm.20124
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发表时间：
2006-05-01
影响因子：
--
通讯作者：
Debec, A
中科院分区：
文献类型：
--
作者：
Karpova, N;Bobinnec, Y;Debec, A
在这项研究中，我们描述了一种新的果蝇蛋白Jupiter，它与包括TAU，MAP2，MAP4在内的几种结构微管相关蛋白（MAPs）具有相同的特性。木星是一种可溶的未折叠分子，具有高净正电荷，富含甘氨酸。它在序列PPGG周围具有两个简并重复序列，由富含丝氨酸的区域隔开。Jupiter在体外与微管结合，并与绿色荧光蛋白（GFP）融合，是体内跟踪微管动力学的极好标记物。在通过“蛋白质陷阱”技术产生的Jupiter转基因果蝇品系中，Jupiter：GFP融合蛋白在发育的不同阶段通过细胞周期定位于微管网络。我们发现特别高的木星：绿色荧光蛋白浓度在年轻的胚胎，幼虫神经系统，前体的眼睛光感受器和成年卵巢。此外，从jupiter：gfp胚胎中，我们建立了两个在整个细胞周期中呈现强荧光微管的永久性细胞系。在这些细胞中，Jupiter：GFP融合蛋白的分布在药物秋水仙碱和紫杉醇处理后再现微管行为。木星细胞系和果蝇品系应该是对微管动力学活体分析感兴趣的生物学家的广泛兴趣。
In this study we describe a novel Drosophila protein Jupiter, which shares properties with several structural microtubule-associated proteins (MAPs) including TAU, MAP2, MAP4. Jupiter is a soluble unfolded molecule with the high net positive charge, rich in Glycine. It possesses two degenerated repeats around the sequence PPGG, separated by a Serine-rich region. Jupiter associates with microtubules in vitro and, fused with the green fluorescent protein (GFP), is all excellent marker to follow microtubule dynamics in vivo. In a jupiter transgenic Drosophila strain generated by the "protein-trap" technique, Jupiter:GFP fusion protein localizes to the microtubule network through the cell cycle at the different stages of development. We found particularly high Jupiter:GFP concentrations in the young embryo, larval nervous system, precursors of eye photoreceptors and adult ovary. Moreover, from jupiter:gfp embryos we have established two permanent cell lines presenting strongly fluorescent microtubules during the whole cell cycle. In these cells, the distribution of the Jupiter:GFP fusion protein reproduces microtubule behavior upon treatment by the drugs colchicine and taxol. The jupiter cell lines and fly strain should be of wide interest for biologists interested in ill vivo analysis of microtubule dynamics.