Role of posttranscriptional processes in the regulation of glutathione S-transferase P1 gene expression in human breast cancer cells.
Role of posttranscriptional processes in the regulation of glutathione S-transferase P1 gene expression in human breast cancer cells.
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转录后过程在人乳腺癌细胞谷胱甘肽 S-转移酶 P1 基因表达调节中的作用。
DOI：
10.1006/bbrc.1997.7109
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Morrow,CS
中科院分区：
文献类型：
--
作者：
Jhaveri,MS;Stephens,TE;Morrow,CS
Human glutathioneS-transferase P1 (GSTP1) is normally expressed in estrogen receptor negative (ER−) but not receptor positive (ER+) cultured breast cancer cells. Previous results indicated that posttranscriptional mechanisms may contribute to this differential expression of GSTP1 (J. Biol. Chem.267, 10544–10550, 1992). Here, we have tested the hypothesis that differences in posttranscriptional processing of primary transcripts to mature mRNA or differences in mRNA stability influence the levels of GSTP1 in ER− versus ER+ breast cancer cells. We examined the expression both of the endogenousGSTP1gene and of uniquely designedGSTP1minigenes that were stably transfected into HS578T (ER−) and MCF7 (ER+) cells. In both cell lines,GSTP1transcripts are processed to mature, functional mRNAs. However, GSTP1 mRNA is considerably less stable in MCF7 than in HS578T cells. These results indicate that for a given level ofGSTP1gene transcription, differential mRNA stability will result in higher steady state levels of GSTP1 mRNA in ER−, HS578T than in ER+, MCF7 cells.
