Bone integration capability of nanopolymorphic crystalline hydroxyapatite coated on titanium implants.
Bone integration capability of nanopolymorphic crystalline hydroxyapatite coated on titanium implants.
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DOI：
10.2147/ijn.s28082
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发表时间：
2012
影响因子：
8
通讯作者：
Ogawa T
中科院分区：
文献类型：
--
作者：
Yamada M;Ueno T;Tsukimura N;Ikeda T;Nakagawa K;Hori N;Suzuki T;Ogawa T
羟基磷灰石（HA）涂层钛促进骨-种植体结合的机制在很大程度上是未知的。此外，将纳米结构HA的制造细化到适用于钛植入物的大规模生产工艺的水平是具有挑战性的。本研究报告成功地创建纳米多晶晶体HA微粗糙钛表面使用火焰喷涂和低温煅烧的组合，并测试其生物学能力，以提高骨植入物的整合。在大鼠模型中对喷砂微粗糙钛植入物和喷砂+HA涂层钛植入物进行生物力学和组织形态学分析。的HA是55%的结晶和纳米级针状架构的发展，在各种直径，长度和方向，这导致在70%的表面积增加相比，noncoated microroughened表面。HA不含杂质污染物，钙/磷比为1.66，相当于化学计量HA的钙/磷比。与微粗糙种植体相比，HA涂层种植体在愈合的早期和晚期均一致地增加了骨-种植体整合的强度。HA涂层种植体显示出种植体表面50 μm附近的骨-种植体接触百分比和骨体积增加，骨和种植体表面之间的软组织干预百分比显著降低。相比之下，HA涂层种植体周围50 μm边界外的骨体积较低。因此，本研究表明，将纯纳米多晶晶体HA添加到微粗糙钛中不仅加速而且提高了骨-植入物整合的水平，并确定了由HA涂层调节的特定组织形态发生参数。特别是，纳米晶HA被证明在增加骨传导性和抑制软组织浸润方面是剧烈的，但效果仅限于植入物周围的直接微环境。
The mechanism by which hydroxyapatite (HA)-coated titanium promotes bone–implant integration is largely unknown. Furthermore, refining the fabrication of nano-structured HA to the level applicable to the mass production process for titanium implants is challenging. This study reports successful creation of nanopolymorphic crystalline HA on microroughened titanium surfaces using a combination of flame spray and low-temperature calcination and tests its biological capability to enhance bone–implant integration. Sandblasted microroughened titanium implants and sandblasted + HA-coated titanium implants were subjected to biomechanical and histomorphometric analyses in a rat model. The HA was 55% crystallized and consisted of nanoscale needle-like architectures developed in various diameters, lengths, and orientations, which resulted in a 70% increase in surface area compared to noncoated microroughened surfaces. The HA was free from impurity contaminants, with a calcium/phosphorus ratio of 1.66 being equivalent to that of stoichiometric HA. As compared to microroughened implants, HA-coated implants increased the strength of bone–implant integration consistently at both early and late stages of healing. HA-coated implants showed an increased percentage of bone–implant contact and bone volume within 50 μm proximity of the implant surface, as well as a remarkably reduced percentage of soft tissue intervention between bone and the implant surface. In contrast, bone volume outside the 50 μm border was lower around HA-coated implants. Thus, this study demonstrated that the addition of pure nanopolymorphic crystalline HA to microroughened titanium not only accelerates but also enhances the level of bone–implant integration and identified the specific tissue morphogenesis parameters modulated by HA coating. In particular, the nanocrystalline HA was proven to be drastic in increasing osteoconductivity and inhibiting soft tissue infiltration, but the effect was limited to the immediate microenvironment surrounding the implant.