CXCL6 promotes human hepatocyte proliferation through the CXCR1-NFκB pathway and inhibits collagen I secretion by hepatic stellate cells
CXCL6 promotes human hepatocyte proliferation through the CXCR1-NFκB pathway and inhibits collagen I secretion by hepatic stellate cells
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CXCL6通过CXCR1-NF kappa B途径促进人肝细胞增殖并抑制肝星状细胞分泌I型胶原
DOI：
10.1139/bcb-2015-0136
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发表时间：
2016-06-01
影响因子：
2.9
通讯作者：
Lu, Lungen
中科院分区：
文献类型：
--
作者：
Li, Zhenghong;Zhang, Qidi;Lu, Lungen
肝细胞增殖和I型胶原（COLI）分泌是肝再生过程中的重要过程。本研究旨在探讨CXCL 6在肝细胞增殖和COLI分泌中的作用。检测慢性B型肝炎（CH B）患者血清CXCL 6水平，并观察CXCL 6对L 0 2肝细胞增殖和L X 2人肝星状细胞分泌COLI的影响。结果发现，慢性乙型肝炎患者血清CXCL 6水平随病情进展而逐渐升高，且血清CXCL 6水平与丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）呈正相关。在体外，CXCL 6促进L02增殖，但这在CXCR 1敲低后被阻断。在L02细胞中，磷酸化I κ B α的水平被CXCL 6上调，但被CXCR 1 siRNA下调。CXCL 6依赖于CXCR 1和CXCR 2抑制LX 2细胞分泌COLI。综上所述，这些数据表明，在CH B过程中CXCL 6的表达增加可以通过CXCR 1-NF κ B途径促进肝细胞增殖，并抑制肝星状细胞分泌COLI。
Hepatocyte proliferation and collagen I (COLI) secretion are important processes during liver regeneration. This study aimed to investigate the role of CXCL6 in hepatocyte proliferation and COLI secretion. Serum CXCL6 levels in patients with chronic hepatitis B (CHB) were examined and the effects of CXCL6 on the proliferation of L02 hepatocytes and the secretion of COLI from LX2 human hepatic stellate cells were evaluated. We found that serum CXCL6 levels increased gradually with disease progression of CHB, and there was positive correlation between serum CXCL6 level and alanine transaminase (ALT) and aspartate transaminase (AST). In vitro, CXCL6 promoted L02 proliferation but this was blocked upon CXCR1 knockdown. The level of phospho-I kappa B alpha was upregulated by CXCL6 but downregulated by CXCR1 siRNA in L02 cells. CXCL6 inhibited the secretion of COLI by LX2 cells, dependent on CXCR1 and CXCR2. Taken together, these data suggest that increased expression of CXCL6 during CHB could promote hepatocyte proliferation through the CXCR1-NF kappa B pathway and inhibit the secretion of COLI by hepatic stellate cells.