Mutagenesis from meiotic recombination is not a primary driver of sequence divergence between Saccharomyces species.
Mutagenesis from meiotic recombination is not a primary driver of sequence divergence between Saccharomyces species.
复制标题
减数分裂重组引起的诱变并不是酵母菌属物种之间序列差异的主要驱动因素。
DOI：
10.1093/molbev/msn186
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发表时间：
2008
影响因子：
10.7
通讯作者：
Noor,MohamedAF
中科院分区：
文献类型：
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作者：
Noor,MohamedAF
许多物种的局部重组率与 DNA 序列多样性呈正相关。为了测试这种关系是否源于减数分裂重组的致突变性，研究经常寻找局部重组率与物种之间序列“分歧”之间的类似关联。由于重组在酵母中具有诱变性，因此我通过测试酵母属物种之间的非编码 DNA 序列差异是否与源自酿酒酵母的减数分裂双链 DNA 断裂或交叉率的测量相关来评估该测定。与预期相反，我发现序列分歧与双链断裂和交叉率要么不相关，要么负相关。提到了一些警告，但这些结果表明减数分裂重组引起的诱变并不是酵母菌种之间序列差异的主要驱动因素。这项研究表明，种间核苷酸分歧和局部重组率之间的关联并不总是重组致突变性的可靠指标。
Local rates of recombination positively correlate with DNA sequence diversity in many species. To test whether this relationship stems from mutagenicity of meiotic recombination, studies often look for a similar association between local rates of recombination and sequence “divergence” between species. Because recombination is mutagenic in yeast, I evaluate this assay by testing whether noncoding DNA sequence divergence betweenSaccharomycesspecies is related to measures of meiotic double-strand DNA breaks or crossover rates derived fromSaccharomycescerevisiae. Contrary to expectation, I find that sequence divergence is either uncorrelated or negatively correlated with rates of both double-strand break and crossover. Several caveats are mentioned, but these results suggest that mutagenesis from meiotic recombination is not the primary driver of sequence divergence betweenSaccharomycesspecies. This study demonstrates that the association between interspecies nucleotide divergence and local recombination rates is not always a reliable indicator of recombination's mutagenicity.