Glioblastoma single extracellular vesicle analysis profiles: wading into new oceans of tumor data.
Glioblastoma single extracellular vesicle analysis profiles: wading into new oceans of tumor data.
复制标题
胶质母细胞瘤单个细胞外囊泡分析概况：涉足肿瘤数据的新海洋。
DOI：
10.1093/neuonc/noz045
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发表时间：
2019
期刊：
影响因子：
15.9
通讯作者：
Jones,Jennifer
中科院分区：
文献类型：
--
作者：
Jones,Jennifer
Within little more than a decade since the earliest reports of glioblastoma (GBM) microvesicles as mediators of the malignant process with diagnostic value, 1, 2 the molecular composition of these extracellular vesicles (EVs) has now been investigated at the level of individual vesicles with single EV analysis (SEA) fluorescence microscopy (this issue). 3 This step forward represents significant progress toward an ultimate goal of the field, which is to be able to detect and analyze tumor-associated EVs, their surface markers, and constituent cargo in assays that are expected to provide insight into tumor phenotype, prognosis, and response to treatment.Many experimental assays have been used in order to isolate and characterize EVs as bulk populations. However, bulk EV assays that interrogate the composition of EVs in patient biofluids lack sufficient sensitivity and specificity to precisely interrogate the composition of EVs that originate from different cell types. In other words, EVs from endothelial cells or platelets are expected to carry cargo (protein or RNA) that reflects the state of a patient’s vascular system more directly than would the cargo carried by EVs from the patient’s intestinal epithelial cells, for example. Molecular signatures of GBM-derived EVs in plasma are similarly likely to be more informative than molecular signatures of the bulk plasma EV populations for GBM-related studies. Following this logic, Fraser et al investigated whether plasma EVs with tumor-associated markers carry informative cargo in GBM patients. The authors captured EVs using a biotin-streptavidin approach, wherein the EVs were conjugated with an average of 5 biotinylated sites per EV and then immobilized on streptavidin-coated glass, for further characterization by immunofluorescence. In the study by Fraser et al, EVs were stratified as having GBM-associated markers (epidermal growth factor receptor [EGFR], EGFR variant [v] III, or epithelial cell adhesion molecule) versus being without those markers, and additional immunofluorescence assays were performed in order to determine the surface molecular repertoire of populations of GBM-derived EVs that can be evaluated with this assay. EV populations were also stratified as large
