Effects of hypoxia on progranulin expression in HT22 mouse hippocampal cells
Effects of hypoxia on progranulin expression in HT22 mouse hippocampal cells
复制标题
缺氧对HT22小鼠海马细胞颗粒体蛋白前体表达的影响
DOI：
10.3892/mmr.2014.2016
复制
发表时间：
2014-05-01
影响因子：
3.4
通讯作者：
Xu, Jie
中科院分区：
文献类型：
--
作者：
Luo, Li;Lu, Lanhai;Xu, Jie
本研究探讨了缺氧对HT 22小鼠海马细胞颗粒蛋白前体表达的影响。为了研究颗粒蛋白前体（PGRN）及其在缺氧后表达的改变，用不同浓度的连二亚硫酸钠（Na 2S 2 O 4; 1-20 mM）处理HT 22细胞固定时间（6 h）或用固定浓度（5 mM）处理不同时间长度（2-10 h）。用免疫细胞化学和western blot分析缺氧后HT 22细胞PGRN的表达。数据显示HT 22细胞能够表达PGRN。双标记结果显示PGRN能够与神经元标记物β III-微管蛋白和doublecortin共定位。蛋白质印迹分析表明，缺氧是能够降低PGRN水平在6小时的浓度依赖性的方式，虽然有一个较短的时间内边际增加。本研究的结果表明，缺氧损伤能够显着影响PGRN的表达，从而提供了新的见解，PGRN在缺血性脑损伤中的作用。
The present study evaluated the effect of hypoxia on the expression of progranulin in HT22 mouse hippocampal cells. To investigate progranulin (PGRN) and the alterations in its expression following hypoxia, the HT22 cells were treated with various concentrations of sodium hydrosulfite (Na2S2O4; 1-20 mM) for a fixed time (6 h) or with a fixed concentration (5 mM) for different lengths of time (2-10 h). The expression of PGRN in the HT22 cells following hypoxia was analyzed by an immunocytochemistry assay and western blot analysis. The data revealed that the HT22 cells were capable of expressing PGRN. Double labeling results revealed that PGRN was able to co-localize with the neuronal markers, beta III-tubulin and doublecortin. Western blot analyses indicated that hypoxia was able to decrease the PGRN levels in a concentration-dependent manner at 6 h, although there was a marginal increase within a shorter period. The results of this study revealed that hypoxic injury is capable of significantly affecting the expression of PGRN, thereby it may provide novel insights with regard to the role of PGRN in ischemic brain injury.