Reduced normogastric electrical activity associated with emesis: A telemetric study in ferrets
Reduced normogastric electrical activity associated with emesis: A telemetric study in ferrets
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DOI：
10.3748/wjg.15.6034
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发表时间：
2009-12-28
影响因子：
4.3
通讯作者：
Andrews, Paul L. R.
中科院分区：
文献类型：
--
作者：
du Sert, Nathalie Percie;Chu, Kit M.;Andrews, Paul L. R.
目的：描述胃肌电活动（GMA）和腹内压变化引起的呕吐刺激（阿扑吗啡和顺铂）在ferret.METHODS：GMA和腹内压记录在清醒的，不受限制的雪貂手术植入无线电遥测发射机。用阿扑吗啡（0.25 mg/kg sc）和顺铂（10 mg/kg ip）攻击动物，分别通过直接观察和腹腔内压力记录1 h和4 h定量呕吐反应。通过频谱分析对GMA进行分析;用于表征GMA的参数是主频率（DF）和频谱功率在心动过缓、正常和心动过速频率范围内的再分配。在腹内压描记图上识别出的回缩为持续时间为0.30 +/- 1.01 s的峰值和幅度为59.57 +/- 2.74 mmHg，呕吐峰时间较长（0.82 ± 0.06 s，P < 0.01），呕吐压力较高（87.73 ± 8.12 mmHg，P < 0.001）。通过直接观察[阿扑吗啡：65.5 +/- 11.8次干呕+呕吐（R+V），顺铂：202.6 +/- 64.1 R+V]和腹内压定量干呕和呕吐次数（阿扑吗啡：68.3 +/- 13.7 R+V，n = 8;顺铂：219.0 +/- 69.2 R+V，n = 8）相关（r = 0.97，P < 0.0001），两种方法的呕吐时间一致。阿扑吗啡使正常胃排空率从45.48% +/- 4.35%下降到36.70 +/- 4.34%（n = 8，P < 0.05），但慢波的DF没有改变[8.95 +/- 0.25 cpm vs 8.68 +/- 0.35 cpm，n = 8，P > 0.05]。顺铂诱导胃功能正常从55.83% +/- 4.30%降低至29.22% +/- 5.16%，胃功能迟缓从14.28% +/- 2.32%增加至31.19% +/- 8.33%（n = 8，P < 0.001），但DF（9.14 ± 0.13 cpm）保持不变（P > 0.05）。顺铂引起的GMA变化先于呕吐反应，呕吐发作前1 h胃排空减少（57.61% ± 5.66%至39.91% ± 5.74%，n = 6，P < 0.05）。围呕吐分析表明，GMA显着扰乱期间和之后立即，但不是立即之前，呕吐epissions.CONCLUSION：呕吐的诱导是可靠的与中断GMA，但也可能发生变化之前和之后的呕吐反应。(C)2009 WJG出版社和百世登。All rights reserved.
AIM: To characterize the gastric myoelectric activity (GMA) and intra-abdominal pressure changes induced by emetic stimuli (apomorphine and cisplatin) in the ferret.METHODS: GMA and intra-abdominal pressure were recorded in conscious, unrestrained ferrets surgically implanted with radiotelemetry transmitters. Animals were challenged with apomorphine (0.25 mg/kg sc) and cisplatin (10 mg/kg ip), and the emetic response was quantified via direct observation and intra-abdominal pressure recording for 1 and 4 h, respectively. The GMA was analyzed by spectral analysis; the parameters used to characterize the GMA were the dominant frequency (DF) and the repartition of spectral power in the bradygastric, normogastric and tachygastric frequency ranges.RESULTS: Retches were identified on the intra-abdominal pressure trace as peaks 0.30 +/- 1.01 s in duration and 59.57 +/- 2.74 mmHg in amplitude, vomit peaks were longer (0.82 +/- 0.06 s, P < 0.01) and reached a higher pressure (87.73 +/- 8.12 mmHg, P < 0.001). The number of retches and vomits quantified via direct observation [apomorphine: 65.5 +/- 11.8 retches + vomits (R+V), cisplatin: 202.6 +/- 64.1 R+V] and intra-abdominal pressure (apomorphine: 68.3 +/- 13.7 R+V, n = 8; cisplatin: 219.0 +/- 69.2 R+V, n = 8) were correlated (r = 0.97, P < 0.0001) and the timing of emesis was consistent between the 2 methods. Apomorphine induced a decrease in normogastria from 45.48% +/- 4.35% to 36.70 +/- 4.34% (n = 8, P < 0.05) but the DF of the slow waves was not changed [8.95 +/- 0.25 counts/min (cpm) vs 8.68 +/- 0.35 cpm, n = 8, P > 0.05]. Cisplatin induced a decrease in normogastria from 55.83% +/- 4.30% to 29.22% +/- 5.16% and an increase in bradygastria from 14.28% +/- 2.32% to 31.19% +/- 8.33% (n = 8, P < 0.001) but the DF (9.14 +/- 0.13 cpm) remained unchanged (P > 0.05). The GMA changes induced by cisplatin preceded the emetic response as normogastria was reduced for 1 h before the onset of emesis (57.61% +/- 5.66% to 39.91% +/- 5.74%, n = 6, P < 0.05). Peri-emesis analysis revealed that the GMA was significantly disturbed during and immediately after, but not immediately before, the emetic episodes.CONCLUSION: The induction of emesis is reliably associated with a disrupted GMA, but changes may also occur prior to and following the emetic response. (C) 2009 The WJG Press and Baishideng. All rights reserved.