Fluorescent gold nanoclusters based photoelectrochemical sensors for detection of H2O2 and glucose
Fluorescent gold nanoclusters based photoelectrochemical sensors for detection of H2O2 and glucose
复制标题
基于荧光金纳米团簇的光电化学传感器用于检测 H2O2 和葡萄糖
DOI：
10.1016/j.bios.2014.08.037
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发表时间：
2015-05-15
影响因子：
12.6
通讯作者：
Yue, Zhao
中科院分区：
文献类型：
--
作者：
Zhang, Jianxiu;Tu, Liping;Yue, Zhao
在这项工作中，开发了基于低毒性荧光金纳米簇（AuNCs）的光电电化学传感器，用于检测H2O2和葡萄糖。本文介绍了传感器的制作工艺以及传感器在不同条件下的光电化学性能。基于AuNCs的能带能级和电子隧穿过程，建立了详细的光电传感模型。设计的传感器随后用于H2O2和葡萄糖检测，无需对aunc进行任何额外的修饰或复合酶固定化。结果表明，基于它们的荧光和催化能力，aunc允许感应H2O2。事实上，我们观察到H2O2被AuNCs催化，并被激发的AuNCs产生的光诱导电子还原。此外，随着H2O2和葡萄糖浓度的增加，光电流振幅也随之增强。研究了aunc周围配体类型和外加电位对输出光电流的影响，优化了测量条件。在500 μ M时，MUA-AuNCs的灵敏度为4.33 nA/mM， LOD为35 μ M。实验结果表明，aunc不仅可以作为一种很有前途的光电材料用于构建光电电化学生物传感器，而且可以作为H2O2传感的催化剂。(C) 2014 Elsevier B.V.版权所有
In this work, low-toxicity fluorescent gold nanoclusters (AuNCs) based photoelectrochemical sensors were developed for H2O2 and glucose detection. Herein, the processes used to fabricate the sensors and the photoelectrochemical performances of the sensors under different conditions were presented. Based on the energy band levels of the AuNCs and electron tunneling processes, a detailed photoelectrochemical sensing model was given. The designed sensors were then used for H2O2 and glucose detection without any extra modification of the AuNCs or complex enzyme immobilization. The results demonstrate that the AuNCs allow for H2O2 sensing based on their capacity for both fluorescence and catalysis. Indeed, it was observed that H2O2 was catalyzed by the AuNCs and reduced by photoinduced electrons derived from excited AuNCs. Furthermore, an enhancement in photocurrent amplitude followed the increase in the concentrations of H2O2 and glucose. The effects of the types of ligands surrounding the AuNCs and the applied potential on the output photocurrent were well studied to optimize the measurement conditions. The sensitivity and LOD of MUA-AuNCs at 500 my were 4.33 nA/mM and 35 mu M, respectively. All experimental results indicated that AuNCs could not only serve as a promising photoelectrical material for building the photoelectrochemical biosensors but as catalysts for H2O2 sensing. (C) 2014 Elsevier B.V. All rights reserved.