Nanoparticle targeting to diseased vasculature for imaging and therapy.
Nanoparticle targeting to diseased vasculature for imaging and therapy.
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DOI：
10.1016/j.nano.2014.02.002
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发表时间：
2014-07
影响因子：
5.4
通讯作者：
Vyavahare, Naren
中科院分区：
文献类型：
--
作者：
Sinha, Aditi;Shaporev, Aleksey;Nosoudi, Nasim;Lei, Yang;Vertegel, Alexey;Lessner, Susan;Vyavahare, Naren
在将药物靶向病变血管系统方面仍然存在重大挑战;最重要的是具有高剪切力的快速血流、稳定靶标的有限可用性以及细胞标志物的异质性和再循环。我们开发了纳米颗粒（NPs），以靶向降解的弹性膜，这是血管疾病中一致的病理特征。体外器官和细胞培养实验表明，这些NP不被细胞吸收，而是保留在细胞外空间内; NP结合与弹性膜损伤的程度成比例。利用三种成熟的血管疾病啮齿动物模型，如主动脉瘤（大鼠中氯化钙介导的主动脉损伤），动脉粥样硬化（脂肪喂养的apoE−/−小鼠）和血管钙化（大鼠中华法林+维生素K注射），我们显示了精确的NP空间靶向降解的血管弹性膜，同时保留健康的血管系统时，NP全身递送。靶向降解的弹性膜的纳米颗粒对于将治疗剂或成像剂递送到患病的脉管系统是有吸引力的。
Significant challenges remain in targeting drugs to diseased vasculature; most important being rapid blood flow with high shear, limited availability of stable targets, and heterogeneity and recycling of cellular markers. We developed nanoparticles (NPs) to target degraded elastic lamina, a consistent pathological feature in vascular diseases. In-vitro organ and cell culture experiments demonstrated that these NPs were not taken up by cells, but instead retained within the extracellular space; NP binding was proportional to the extent of elastic lamina damage. With three well-established rodent models of vascular diseases such as aortic aneurysm (calcium chloride mediated aortic injury in rats), atherosclerosis (fat-fed apoE−/− mice), and vascular calcification (warfarin + vitamin K injections in rats), we show precise NPs spatial targeting to degraded vascular elastic lamina while sparing healthy vasculature when NPs were delivered systemically. Nanoparticle targeting degraded elastic lamina is attractive to deliver therapeutic or imaging agents to the diseased vasculature.
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影响因子：
158.5
作者：
KAMINSKI, MS;ZASADNY, KR;WAHL, RL
通讯作者：
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影响因子：
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DOI：
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发表时间：
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影响因子：
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作者：
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