Analysis of pollutant-induced changes in mitochondrial DNA methylation.
Analysis of pollutant-induced changes in mitochondrial DNA methylation.
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分析污染物诱导的线粒体DNA甲基化变化。
DOI：
10.1007/978-1-4939-2288-8_19
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Barrow, Timothy M
中科院分区：
文献类型：
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作者：
Byun, Hyang-Min;Barrow, Timothy M
越来越多的证据表明，暴露于空气污染物与人类疾病有关，并可能通过核基因组的表观遗传修饰起作用，但很少有出版物描述其对线粒体基因组的影响。线粒体DNA可能更容易受到污染物诱导的变化，通过增加细胞中的氧化应激，因此这一领域的研究越来越感兴趣。许多用于研究核基因组DNA甲基化的技术也适用于线粒体表观遗传学研究。在这一章中，我们描述了一个协议的线粒体DNA从外周血样本的分离和5-甲基胞嘧啶含量的亚硫酸氢盐焦磷酸测序分析。
There is increasing evidence that exposure to air pollutants are associated with human disease and may act through epigenetic modification of the nuclear genome, but there have been few publications describing their impact upon the mitochondrial genome. Mitochondrial DNA may be more susceptible to pollutant-induced changes via increased oxidative stress in the cell, and therefore this field of research is of growing interest. Many techniques employed to study DNA methylation of the nuclear genome are also applicable to mitochondrial epigenetic studies. In this chapter, we describe a protocol for the isolation of mitochondrial DNA from peripheral blood samples and the analysis of 5-methlycytosine content by bisulfite-pyrosequencing.