Combination of Root Surface Modification with BMP-2 and Collagen Hydrogel Scaffold Implantation for Periodontal Healing in Beagle Dogs.
Combination of Root Surface Modification with BMP-2 and Collagen Hydrogel Scaffold Implantation for Periodontal Healing in Beagle Dogs.
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DOI：
10.2174/1874210601509010052
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Kawanami M
中科院分区：
文献类型：
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作者：
Kato A;Miyaji H;Ishizuka R;Tokunaga K;Inoue K;Kosen Y;Yokoyama H;Sugaya T;Tanaka S;Sakagami R;Kawanami M
目的：牙根表面的生物修饰在牙周创面愈合中起着重要作用。用骨形态发生蛋白(BMP)对牙根表面进行修饰可以刺激骨和牙骨质样组织的形成，但同时也会观察到严重的强直。因此，生物安全的胶原水凝胶支架可能有助于提供牙周韧带细胞和预防强直。我们观察了骨形态发生蛋白修饰联合胶原水凝胶支架植入对狗牙周创面愈合的影响。材料和方法：胶原水凝胶支架由I型胶原海绵和胶原水凝胶组成。对6只Beagle犬进行了单壁骨下缺损(深5 mm，宽3 mm)的手术修复。骨形态发生蛋白/胶原组：将骨形态发生蛋白-2涂抹于根面(负荷量：L 1µg/µg)，并用胶原水凝胶支架充填。BMP组或Col组进行BMP-2涂层或支架植入。术后4周评价组织计量学参数。结果：单用BMP可促进牙槽骨形成和牙槽骨强直。相反，BMP/Col组经常促进牙周附着的重建，包括牙骨质样组织、牙周膜和牙槽骨。与所有其他组相比，BMP/Col组的新牙周韧带数量明显增加。此外，在BMP/Col组中很少观察到强直。结论：BMP表面修饰与胶原水凝胶支架植入相结合的方法有利于牙周附着的重建。植入胶原水凝胶可抑制骨形态发生蛋白相关的强直。
Objective : Biomodification of the root surface plays a major role in periodontal wound healing. Root surface modification with bone morphogenetic protein (BMP) stimulates bone and cementum-like tissue formation; however, severe ankylosis is simultaneously observed. Bio-safe collagen hydrogel scaffolds may therefore be useful for supplying periodontal ligament cells and preventing ankylosis. We examined the effects of BMP modification in conjunction with collagen hydrogel scaffold implantation on periodontal wound healing in dogs. Material and Methods: The collagen hydrogel scaffold was composed of type I collagen sponge and collagen hydrogel. One-wall infrabony defects (5 mm in depth, 3 mm in width) were surgically created in six beagle dogs. In the BMP/Col group, BMP-2 was applied to the root surface (loading dose; 1 µg/µl), and the defects were filled with collagen hydrogel scaffold. In the BMP or Col group, BMP-2 coating or scaffold implantation was performed. Histometric parameters were evaluated at 4 weeks after surgery. Results: Single use of BMP stimulated formation of alveolar bone and ankylosis. In contrast, the BMP/Col group frequently enhanced reconstruction of periodontal attachment including cementum-like tissue, periodontal ligament and alveolar bone. The amount of new periodontal ligament in the BMP/Col group was significantly greater when compared to all other groups. In addition, ankylosis was rarely observed in the BMP/Col group. Conclusion: The combination method using root surface modification with BMP and collagen hydrogel scaffold implantation facilitated the reestablishment of periodontal attachment. BMP-related ankylosis was suppressed by implantation of collagen hydrogel.