Genomic organization of the retinoic acid receptor gamma gene.
Genomic organization of the retinoic acid receptor gamma gene.
复制标题
视黄酸受体γ基因的基因组结构。
DOI：
10.1093/nar/19.3.573
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发表时间：
1991
影响因子：
14.9
通讯作者：
Pfahl,M
中科院分区：
文献类型：
--
作者：
Lehmann,JM;Hoffmann,B;Pfahl,M
视黄酸受体（RAR）属于配体应答基因调控蛋白大家族，包括类固醇和甲状腺激素受体。这些蛋白质含有两个高度保守的结构域，参与决定它们的DNA和配体结合活性。已经鉴定了三种不同的RAR（RARα、RAR β和RAR γ），它们由不同染色体上的基因编码。已经描述了三种受体的其他同种型，它们在N-末端区域都不同。为了深入了解RAR异构体产生的基因组组织和机制，我们分析了RARγ基因的基因组结构。RARγ蛋白的主要部分（包括DNA和配体结合结构域）由7个外显子编码，这些外显子对于所有RARg亚型都是相同的，并且由RARγ基因的相对较小部分代表。该基因的主要部分编码γ1和γ2同种型的单独N-末端外显子和γ1非翻译区的几个外显子。我们发现RARγ2的转录受其自身启动子的调控。与类固醇受体亚家族相比，RARγ的各种剪接位点发生在改变的位置，这表明RAR亚家族在进化早期已经分化。
The retinoic acid receptors (RAR) belong to the large family of ligand responsive gene regulatory proteins that includes receptors for steroid and thyroid hormones. These proteins contain two highly conserved domains, involved In determining their DNA and ligand binding activities. Three distinct RARs have been identified (RARα,β, andγ) which are encoded by genes on separate chromosomes. Additional isoforms of the three receptors have been described that all differ in the N-terminal regions. To gain insight Into the genomic organization and mechanisms of RAR isoform generation, we have analyzed the genomic structure of the RARγgene. The major portion of the RARγprotein, including DNA and ligand binding domains, is encoded by seven exons that are identical for all RARg Isoforms and are represented by a relatively small portion of the RARγgene. The major portion of this gene encodes separate N-terminal exons forγ1 andγ2 isoforms and several exons forγ1 untranslated regions. We show that RARγ2 transcription Is regulated by its own promoter. In comparison with the steroid receptor subfamily, various splice sites of RARγoccur at altered positions, suggesting that the RAR subfamily has diverged early during evolution.