Arabidopsis SWR1-associated protein methyl-CpG-binding domain 9 is required for histone H2A.Z deposition
Arabidopsis SWR1-associated protein methyl-CpG-binding domain 9 is required for histone H2A.Z deposition
复制标题
拟南芥 SWR1 相关蛋白甲基 CpG 结合域 9 是组蛋白 H2A.Z 沉积所必需的
DOI：
10.1038/s41467-019-11291-w
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发表时间：
2019-07-26
影响因子：
16.6
通讯作者：
Jacobsen, Steven E.
中科院分区：
文献类型：
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作者：
Potok, Magdalena E.;Wang, Yafei;Jacobsen, Steven E.
组蛋白变异体H2A.Z由SWI2/SNF2相关的1染色质重塑复合体(SWR1-C)沉积，对于真核生物的基因调控是重要的，但拟南芥SWR1-C的组成尚未完全确定。在这里，我们旨在寻找一个保守的拟南芥SWR1亚单位肌动蛋白相关蛋白6(ARP6)的相互作用伙伴。我们分离了9个预测的成分，并确定了与组蛋白乙酰化和染色质生物学有关的其他相互作用因子。其中，甲基CpG结合域9(MBD9)与模拟开关(ISWI)染色质重塑复合体也有很强的相互作用。MBD9是在依赖ARP6的基因座的不同子集沉积H_2A.Z所必需的。MBD9优先结合到含有高水平激活组蛋白标记的基因5‘端的核小体缺失区域。这些数据表明，MBD9是一种SWR1-C相互作用蛋白，需要在活跃转录基因的子集上沉积H2A.Z。
Deposition of the histone variant H2A.Z by the SWI2/SNF2-Related 1 chromatin remodeling complex (SWR1-C) is important for gene regulation in eukaryotes, but the composition of the Arabidopsis SWR1-C has not been thoroughly characterized. Here, we aim to identify interacting partners of a conserved Arabidopsis SWR1 subunit ACTIN-RELATED PROTEIN 6 (ARP6). We isolate nine predicted components and identify additional interactors implicated in histone acetylation and chromatin biology. One of the interacting partners, methyl-CpG-binding domain 9 (MBD9), also strongly interacts with the Imitation SWItch (ISWI) chromatin remodeling complex. MBD9 is required for deposition of H2A.Z at a distinct subset of ARP6-dependent loci. MBD9 is preferentially bound to nucleosome-depleted regions at the 5’ ends of genes containing high levels of activating histone marks. These data suggest that MBD9 is a SWR1-C interacting protein required for H2A.Z deposition at a subset of actively transcribing genes.