TIGHT GENETIC-LINKAGE BETWEEN THE KDR INSECTICIDE RESISTANCE TRAIT AND A VOLTAGE-SENSITIVE SODIUM-CHANNEL GENE IN THE HOUSE-FLY
TIGHT GENETIC-LINKAGE BETWEEN THE KDR INSECTICIDE RESISTANCE TRAIT AND A VOLTAGE-SENSITIVE SODIUM-CHANNEL GENE IN THE HOUSE-FLY
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DOI：
10.1073/pnas.91.7.2483
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发表时间：
1994-03-29
影响因子：
11.1
通讯作者：
SODERLUND, DM
中科院分区：
文献类型：
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作者：
KNIPPLE, DC;DOYLE, KE;SODERLUND, DM
家蝇（Musca domestica）对kdr杀虫剂的抗性特性使其能够抵抗1,1,1-三氯-2,2-双（对氯苯）乙烷（DDT）和拟除虫菊酯的快速麻痹（击倒）和致死效应。具有kdr特征的果蝇对这些化合物的神经元敏感性降低。它们作用于对电压敏感的神经膜钠通道。为了验证电压敏感钠通道基因突变导致kdr表型的假设，我们克隆了与黑腹果蝇对钠通道基因的家蝇同源片段相对应的基因组DNA，并在kdr菌株538ge和近交的杀虫剂敏感实验室斑点之间的该片段中发现了限制性位点多态性。并开发了一种基于聚合酶链反应的灵敏诊断方法来确定个体蝇的钠通道基因型。利用这些分子标记进行的遗传连锁分析表明，kdr性状与电压敏感钠通道基因片段紧密相连（在约1个图谱单位内），表现出DNA序列多态性。这些发现为电压敏感钠通道基因或附近的突变作为kdr抗性的基础提供了遗传证据。
The kdr insecticide resistance trait in the house fly, Musca domestica, confers resistance to the rapid paralysis (knockdown) and lethal effects of 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT) and pyrethroids. Flies with the kdr trait exhibit reduced neuronal sensitivity to these compounds. which are known to act at voltage-sensitive sodium channels of nerve membranes. To test the hypothesis that a mutation in a voltage-sensitive sodium channel gene confers the kdr phenotype, we have cloned genomic DNA corresponding to a segment of the house fly homologue of the para sodium channel gene of Drosophila melanogaster, identified restriction-site polymorphisms within this segment between the kdr strain 538ge and an inbred insecticide-susceptible lab stain, and developed a sensitive polymerase chain reaction-based diagnostic procedure to determine the sodium channel genotype of individual flies. A genetic linkage analysis performed with these molecular markers shows that the kdr trait is tightly linked (within about 1 map unit) to the voltage-sensitive sodium channel gene segment exhibiting the DNA sequence polymorphism. These findings provide genetic evidence for a mutation at or near a voltage-sensitive sodium channel gene as the basis for kdr resistance.