Advanced Tuneable Micronanoplatforms for Sensitive and Selective Multiplexed Spectroscopic Sensing via Electro-Hydrodynamic Surface Molecular Lithography
Advanced Tuneable Micronanoplatforms for Sensitive and Selective Multiplexed Spectroscopic Sensing via Electro-Hydrodynamic Surface Molecular Lithography
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DOI：
10.1002/advs.202306068
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发表时间：
2024-01-15
期刊：
影响因子：
15.1
通讯作者：
Goldberg Oppenheimer，Pola
中科院分区：
文献类型：
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作者：
Gomes，Paulo De Carvalho;Hin-Chu，Martin;Goldberg Oppenheimer，Pola
材料的微米和纳米图案化是新兴技术的基石之一，已经改变了芯片实验室诊断的研究能力。在此，开发了一种微米和纳米光刻方法，能够在亚微米尺度上结构化材料，这反过来又可以加速小型化平台技术和生物医学传感器的发展。其基础是先进的电流体动力学表面分子光刻技术，通过诱导界面不稳定性产生微米和纳米结构基底，独特地集成了合成表面识别。这种方法能够制造具有可调特征尺寸的设计图案，这些图案通过合成纳米化学进行功能化，用于高灵敏度，选择性，快速的分子传感。高精度压电光刻设备的开发实现了可再现的衬底制造，具有最佳的信号增强，针对每个微米和纳米结构阵列上的捕获分子的功能化进行了优化。这有助于空间分离，这在光谱传感期间，能够实现目标分子的多路测量，从而在微小浓度下建立检测。随后，这种纳米等离子体激元芯片实验室与非常规计算分类算法和表面增强拉曼光谱相结合，旨在解决与疾病指示性生物标志物的及时床旁检测相关的挑战，用于创伤性脑损伤指示性聚糖生物标志物的多重检测的验证试验，证明了用于准确检测的简单且具有成本效益的微米和纳米平台。
Micro‐ and nanopatterning of materials, one of the cornerstones of emerging technologies, has transformed research capabilities in lab‐on‐a‐chip diagnostics. Herein, a micro‐ and nanolithographic method is developed, enabling structuring materials at the submicron scale, which can, in turn, accelerate the development of miniaturized platform technologies and biomedical sensors. Underpinning it is the advanced electro‐hydrodynamic surface molecular lithography, via inducing interfacial instabilities produces micro‐ and nanostructured substrates, uniquely integrated with synthetic surface recognition. This approach enables the manufacture of design patterns withtuneablefeature sizes, which are functionalized via synthetic nanochemistry for highly sensitive, selective, rapid molecular sensing. The development of a high‐precision piezoelectric lithographic rig enables reproducible substrate fabrication with optimum signal enhancement optimized for functionalization with capture molecules on each micro‐ and nanostructured array. This facilitates spatial separation, which during the spectroscopic sensing, enables multiplexed measurement of target molecules, establishing the detection at minute concentrations. Subsequently, this nano‐plasmonic lab‐on‐a‐chip combined with the unconventional computational classification algorithm and surface enhanced Raman spectroscopy, aimed to address the challenges associated with timely point‐of‐care detection of disease‐indicative biomarkers, is utilized in validation assay for multiplex detection of traumatic brain injury indicative glycan biomarkers, demonstrating straightforward and cost‐effective micro‐ and nanoplatforms for accurate detection.