General Strategy for Designing Functionalized Magnetic Microspheres for Different Bioapplications
General Strategy for Designing Functionalized Magnetic Microspheres for Different Bioapplications
复制标题
为不同生物应用设计功能化磁性微球的一般策略
DOI：
10.1021/la901258p
复制
发表时间：
2009-10-06
期刊：
影响因子：
3.9
通讯作者：
Ren, Jun
中科院分区：
文献类型：
--
作者：
Huang, Xinglu;Zhuang, Jie;Ren, Jun
磁性纳米粒子的表面功能化和水溶性是其生物应用的关键。在这里，我们描述了一种合成方法，用于直接制备广泛的功能化和亲水性磁性聚合物颗粒（MPP），这是简单和通用的，涉及使用不同的聚合物作为官能团的来源。这种改变反应中使用的聚合物的简单策略可以产生各种各样的具有大量官能团的亲水性MIP。为了生物应用的目的，我们合成了三种具有典型功能基团的MPPs，如羟基（-OH）、氨基（-NH 2）和羧基（-COOH），并通过透射电子显微镜（TEM）、扫描电子显微镜（SEM）、热重分析（TGA）、X射线粉末衍射（XRD）、拉曼光谱、和傅里叶变换红外（FTIR）光谱。还研究了MPPs的磁饱和度，并且对于大多数生物应用是足够的。使用细胞增殖和凋亡测定显示MPP具有良好的生物相容性。两种具有不同功能基团的MPP成功地用于细胞内成像和抗体纯化。我们的研究结果表明，这种简单而通用的合成策略具有设计亲水性磁性纳米粒子的潜力，具有多功能性，满足一系列的生物应用。
Surface functionalization and water solubility of magnetic nanoparticles are crucial for bioapplication. Here, we describe a synthetic approach for direct preparation of a wide range of functionalized and hydrophilic magnetic polymer particles (MPPs) that is both simple and general and involves using different polymers as the source of functional groups. This simple strategy of changing the polymer used in the reaction can give rise to a wide variety of hydrophilic M Pis with a high number of functional groups. For the purpose of bioapplication, we synthesized three types of MPPs with typical functional groups, such as hydroxyl groups (-OH),amino groups (-NH2), and carboxyl groups (-COOH), and further characterized these MPPs by transmission electronic microscopy (TEM), scanning electronic microscopy (SEM), thermogravimetric analysis (TGA), X-ray powder diffraction (XRD), Raman spectroscopy, and Fourier transform infrared (FTIR) spectroscopy. The magnetic saturation of the MPPs was also studied and was adequate for most bioapplications. MPPs were shown to have good biocompatibility using cell proliferation and apoptosis assays. Two types of MPPs with different functional groups were used successfully for intracellular imaging and antibody purification. Our results demonstrate that this simple and general synthesis strategy has potential for designing hydrophilic magnetic nanoparticles with multifunctionalities that cater for a range of bioapplications.