Mechanism of β-cypermethrin metabolism by Bacillus cereus GW-01
Mechanism of β-cypermethrin metabolism by Bacillus cereus GW-01
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蜡样芽胞杆菌 GW-01 代谢β-氯氰菊酯的机制
DOI：
10.1016/j.cej.2021.132961
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发表时间：
2021-10
影响因子：
15.1
通讯作者：
Gang Liu
中科院分区：
文献类型：
--
作者：
Jiayuan Zhao;Yangdan Jiang;Lanmin Gong;Xiaofeng Chen;Qingling Xie;Yan Jin;Juan Du;Shufang Wang;Gang Liu
土壤和食品中的农药残留对人类健康是一个令人关切的问题。生物降解是减少这些残留物的一个有吸引力的选择，但需要对降解机制有更多的了解，以确保在该过程中不会产生有毒的死端产物。本研究从绵羊瘤胃食糜中分离到一株β-氯氰菊酯（β-CY）降解菌，命名为GW-01。培养液的HPLC分析表明，菌株GW-01在7 d内对100 mg/L β-CY的降解率达60%以上。菌株GW-01在β-CY降解过程中瞬时积累3-苯氧基苯甲酸（3-PBA）、苯酚和苯甲酸。GW-01在β-CY及其代谢产物降解过程中的基因组、转录组和蛋白质组分析提供了证据，证明α/β-水解酶和细胞色素aa 3醌醇氧化酶分别负责β-CY和3-PBA的降解。氯水解酶参与了苯酚向邻苯二酚的降解。转录和蛋白质组学证据也分别被发现上调苯甲酸降解途径和元途径（儿茶酚-2，3-双加氧酶）。基于这些结果，提出了β-CY降解的途径，为微生物降解拟除虫菊酯的机制提供了更深入的了解。
Pesticide residues in soil and food are of concern for human health. Biodegradation is an attractive option for reducing these residues, but more insight into the mechanism of degradation is necessary to ensure that toxic deadend products are not produced in the process. In this study, a β-cypermethrin (β-CY) degrading bacterium which we named GW-01 was isolated from a sheep’s rumen chyme. HPLC analyses of culture extracts revealed that train GW-01 degraded ∼ 60 % of 100 mg/L β‑CY within 7 d. Strain GW-01 transiently accumulated 3-phenoxybenzoic acid (3-PBA), phenol, and benzoic acid during β-CY degradation. Genomic, transcriptomic, and proteomic analyses of GW-01 during degradation of β-CY and its metabolites provided evidence that an α/β- hydrolase and a cytochrome aa3 quinol oxidase were responsible for the degradation of β-CY and 3-PBA, respectively. Chlorohydrolase was implicated in the degradation of phenol to catechol. Transcriptional and proteomic evidence was also found for upregulation of the benzoic acid degradation pathway andmeta-pathway (catechol-2,3-dioxygenase), respectively. Based on these results a pathway for β-CY degradation is propose that provides greater insight into the mechanism of pyrethroid degradation by microorganisms.
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影响因子：
6.8
作者：
Jiayuan Zhao;Dongying Jia;Yuanlong Chi;K. Yao
通讯作者：
Jiayuan Zhao;Dongying Jia;Yuanlong Chi;K. Yao
地衣芽孢杆菌 B-1 和米曲霉 M-4 共培养对β-氯氰菊酯和 3-苯氧基苯甲酸的共代谢降解
影响因子：
3.7
作者：
Jiayuan Zhao;Yuanlong Chi;Yingchao Xu;Dongying Jia;Kai Yao
通讯作者：
Kai Yao
影响因子：
11.4
作者：
Banerjee, Aditi;Ghoshal, Aloke K.
通讯作者：
Ghoshal, Aloke K.
影响因子：
3.4
作者：
Bazinet AL
通讯作者：
Bazinet AL
DOI：
10.1016/j.jksus.2020.08.012
发表时间：
2020-08
期刊：
Journal of King Saud University - Science
影响因子：
--
作者：
Shahid Sher;S. Hussain;A. Rehman
通讯作者：
Shahid Sher;S. Hussain;A. Rehman