Use of Cultured Cells to Study Alcohol Metabolism

Use of Cultured Cells to Study Alcohol Metabolism
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使用培养细胞研究酒精代谢

DOI:
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发表时间:
2006
期刊:
Alcohol Research and Health
影响因子:
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通讯作者:
D. Clemens
D. Clemens
中科院分区:
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文献类型:
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作者:
D. Clemens

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在酒精研究中使用实验室培养的细胞(即培养细胞)有许多优点。其中包括研究个体代谢途径的能力,在没有混杂变量的情况下精确控制乙醇及其代谢物暴露的能力,以及基因相同(即克隆)细胞系的均匀性。此外,由于培养大量细胞的成本和相对容易,可以进行更多的实验复制来证实发现。如本文所述,使用培养细胞极大地促进了对酒精代谢影响细胞并最终导致酒精性肝病的机制的理解。
The use of cells grown in the laboratory (i.e., cultured cells) in alcohol research has many advantages. Among these are the ability to investigate individual metabolic pathways, the ability to precisely control exposure to ethanol and its metabolites in the absence of confounding variables, and the uniformity of genetically identical (i.e., clonal) cell lines. Additionally, because of the cost and relative ease of culturing large quantities of cells, many more experimental replicas may be performed to confirm findings. As described in this article, the use of cultured cells has contributed greatly to the understanding of the mechanisms by which alcohol metabolism affects cells and ultimately results in alcoholic liver disease.
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