Photon spectroscopy by picoseconds differential Geiger-mode Si photomultiplier
Photon spectroscopy by picoseconds differential Geiger-mode Si photomultiplier
复制标题
皮秒差分盖革模式硅光电倍增管的光子光谱
DOI：
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发表时间：
2018
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影响因子：
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通讯作者：
aMIFtek Corp
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文献类型：
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作者：
Masanobu Yamamoto;Keegan Hernandeza;J. P. Robinsona;aMIFtek Corp
像素阵列硅光电倍增管(SiPM)被认为是一种具有皮秒雪崩过程的优秀光子传感器，能够放大数百万个光电子。此外，较高的量子效率(QE)、小尺寸、低偏压、耐光性是生物应用的诱人特征。主要的缺点是由于50 ns的充电过程和高暗计数的有限的动态范围，这是一个额外的障碍。采用超快微分信号处理、热电器件控温和具有9位小数动态范围的千兆光子计数器，研制了一套宽动态硅光子探测系统。测试性能为6个数量级，脉宽为600ps，灵敏度为亚FW。结合405 nm激光照射和车载单色仪，激光诱导荧光光谱仪(LIPS)的扫描范围为200~900 nm，最大扫描速度为500 nm/s，半高宽为1 nm。基于普朗克方程E=hν，该光子计数谱为数字处理光谱分析提供了基础。其优势包括其最终的灵敏度、理论线性以及不使用任意单位的定量和对数分析。激光激发也可用于评估材料在较高能量照射下的光漂白或氧化。传统的典型光电流检测下限约为1pW，其中包含数百万个光子，但使用我们的系统可以评估任何细胞仪或成像系统组件中的光子光谱，并确定光学中的背景噪声和自体荧光(AFL)。此外，光子流数字信号为皮秒时间域分析开辟了一条新途径。光子光谱学是分析生物中荧光和光学性质的一种强有力的方法。
The pixel array silicon photomultiplier (SiPM) is known as an excellent photon sensor with picoseconds avalanche process with the capacity for millions amplification of photoelectrons. In addition, a higher quantum efficiency(QE), small size, low bias voltage, light durability are attractive features for biological applications. The primary disadvantage is the limited dynamic range due to the 50ns recharge process and a high dark count which is an additional hurdle. We have developed a wide dynamic Si photon detection system applying ultra-fast differentiation signal processing, temperature control by thermoelectric device and Giga photon counter with 9 decimal digits dynamic range. The tested performance is six orders of magnitude with 600ps pulse width and sub-fW sensitivity. Combined with 405nm laser illumination and motored monochromator, Laser Induced Fluorescence Photon Spectrometry (LIPS) has been developed with a scan range from 200~900nm at maximum of 500nm/sec and 1nm FWHM. Based on the Planck equation E=hν, this photon counting spectrum provides a fundamental advance in spectral analysis by digital processing. Advantages include its ultimate sensitivity, theoretical linearity, as well as quantitative and logarithmic analysis without use of arbitrary units. Laser excitation is also useful for evaluation of photobleaching or oxidation in materials by higher energy illumination. Traditional typical photocurrent detection limit is about 1pW which includes millions of photons, however using our system it is possible to evaluate the photon spectrum and determine background noise and auto fluorescence(AFL) in optics in any cytometry or imaging system component. In addition, the photon-stream digital signal opens up a new approach for picosecond time-domain analysis. Photon spectroscopy is a powerful method for analysis of fluorescence and optical properties in biology.