Preliminary probiotic and technological characterization of Pediococcus pentosaceus strain KID7 and in vivo assessment of its cholesterol-lowering activity.
Preliminary probiotic and technological characterization of Pediococcus pentosaceus strain KID7 and in vivo assessment of its cholesterol-lowering activity.
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DOI：
10.3389/fmicb.2015.00768
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发表时间：
2015
影响因子：
5.2
通讯作者：
Suh JW
中科院分区：
文献类型：
--
作者：
Damodharan K;Lee YS;Palaniyandi SA;Yang SH;Suh JW
本研究旨在通过体外和体内研究，对戊糖小球菌属菌株KID7的益生菌特性进行研究。该菌株对口腔-胃肠道转运具有耐受性，对Caco-2细胞株具有粘附性，并具有抗菌活性。KID7体外具有胆盐水解酶活性和降胆固醇活性。用致动脉粥样硬化饲料喂养的高胆固醇血症小鼠研究KID7的体内降胆固醇活性。实验动物(C57BL/6J小鼠)分为4组，即正常饲料喂养组(NCD)、致动脉粥样硬化饲料喂养组(HCD)、致动脉粥样硬化饲料加KID7组(HCD-KID7)和致动脉粥样硬化饲料+嗜酸乳杆菌ATCC 43121组(HCD-L.Ac)作为阳性对照。HCD-KID7组血清总胆固醇(T-CHO)水平较HCD组显著降低19.8%(P<；0.05)，而HCD-L.Ac组与HCD组相比无显著差异。HCD-KID7组和HCD-L.ac组的低密度脂蛋白水平分别比HCD组降低35.5%和38.7%(P均<0.05)。HCD-KID7组和HCD-L.ac组谷丙转氨酶(GPT)水平明显低于HCD组，与NCD组相当。HCD-KID7组大鼠肝脏T-CHO水平明显低于HCD组(P<；0.05)，而HCD-L.Ac组无明显变化。肝组织脂代谢相关基因的表达分析显示，高密度脂蛋白受体、胆固醇-7α-羟基酶和载脂蛋白E的基因表达水平明显高于高脂饮食组。此外，KID7在冷冻干燥和随后的结合脱脂牛奶和半乳甘露聚糖的储存条件下表现出理想的生存能力。戊糖假单胞菌KID7可能是一种潜在的益生菌菌株，经过适当的人体临床试验后，可用于开发降胆固醇功能食品。
The study was aimed to characterize the probiotic properties of a Pediococcus pentosaceus strain, KID7, by in vitro and in vivo studies. The strain possessed tolerance to oro-gastrointestinal transit, adherence to the Caco-2 cell line, and antimicrobial activity. KID7 exhibited bile salt hydrolase activity and cholesterol-lowering activity, in vitro. In vivo cholesterol-lowering activity of KID7 was studied using atherogenic diet-fed hypercholesterolemic mice. The experimental animals (C57BL/6J mice) were divided into 4 groups viz., normal diet-fed group (NCD), atherogenic diet-fed group (HCD), atherogenic diet- and KID7-fed group (HCD-KID7), and atherogenic diet- and Lactobacillus acidophilus ATCC 43121-fed group (HCD-L.ac) as positive control. Serum total cholesterol (T-CHO) level was significantly decreased by 19.8% in the HCD-KID7 group (P < 0.05), but not in the HCD-L.ac group compared with the HCD group. LDL cholesterol levels in both HCD-KID7 and HCD-L.ac groups were decreased by 35.5 and 38.7%, respectively, compared with HCD group (both, P < 0.05). Glutamyl pyruvic transaminase (GPT) level was significantly lower in the HCD-KID7 and HCD-L.ac groups compared to HCD group and was equivalent to that of the NCD group. Liver T-CHO levels in the HCD-KID7 group were reduced significantly compared with the HCD group (P < 0.05) but not in the HCD-L.ac group. Analysis of expression of genes associated with lipid metabolism in liver showed that low-density lipoprotein receptor (LDLR), cholesterol-7α-hydroxylase (CYP7A1) and apolipoprotein E (APOE) mRNA expression was significantly increase in the HCD-KID7 group compared to the HCD group. Furthermore, KID7 exhibited desired viability under freeze-drying and subsequent storage conditions with a combination of skim milk and galactomannan. P. pentosaceus KID7 could be a potential probiotic strain, which can be used to develop cholesterol-lowering functional food after appropriate human clinical trials.