Immunochemical detection of multiple conformations within a 36 base pair oligonucleotide.
Immunochemical detection of multiple conformations within a 36 base pair oligonucleotide.
复制标题
36 碱基对寡核苷酸内多种构象的免疫化学检测。
DOI：
10.1093/nar/16.22.10643
复制
发表时间：
1988
影响因子：
14.9
通讯作者：
Stollar,BD
中科院分区：
文献类型：
--
作者：
Sanford,DG;Kotkow,KJ;Stollar,BD
合成了一个36碱基对的嵌合寡核苷酸，其两端各有一个DNA双链中心，两端为RNA/DNA杂交体。这些不同的寡核苷酸区域采用不同的构象，这些构象是用抗体探针检测的。采用酶联免疫吸附试验(ELISA)和凝胶电泳法检测识别B-DNA、Z-DNA和RNA/DNA杂合体的抗体结合情况。该寡核苷酸的DNA双链核心在0.14M的氯化钠溶液中为B-构象。在高盐溶液(4M氯化钠)中，DNA核心呈Z-构象。寡聚体末端的RNA/DNA杂化采用不同于B-DNA和A-RNA的构象。
A 36 base pair chimeric oligonucleotide containing a central core of DNA duplex flanked by RNA/DNA hybrid at each end was synthesized. These distinct regions of the oligonucleotide adopt different conformations which were detected with antibody probes. Enzyme linked immunosorbent assays (ELISA) and a gel electrophoresis retardation assay were used to demonstrate the binding of antibodies which recognize B-DNA, Z-DNA and RNA/DNA hybrid. The DNA duplex core of this oligonucleotide adopts the B-conformation in 0.14 M NaCl. In high salt solution (4 M NaCl) the DNA core adopts the Z-conformation. The RNA/DNA hybrid at the ends of the oligomer adopt a conformation which is distinct from both B-DNA and A-RNA.