Enhancement of Penaeus monodon shrimp postlarvae growth and survival without water exchange using marine Bacillus pumilus and periphytic microalgae
Enhancement of Penaeus monodon shrimp postlarvae growth and survival without water exchange using marine Bacillus pumilus and periphytic microalgae
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DOI：
10.1007/s12562-010-0230-x
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发表时间：
2010-05-01
影响因子：
1.9
通讯作者：
Yusoff, Fatimah Md.
中科院分区：
文献类型：
--
作者：
Banerjee, Sanjoy;Khatoon, Helena;Yusoff, Fatimah Md.
我们研究了在孵化场系统中使用海洋细菌和周围生长微藻来改善水质和提高单对虾生长和存活率的可能性。在不改变培养水的情况下，评价了3种处理对单斑单胞芽孢杆菌后幼虫（PL）的影响：单独使用短小芽孢杆菌(B)；周边微藻(M)；B. pumilus +周边微藻（BM）。在不含细菌和周边微藻的不变水池中培养单胞假单胞假体PL作为对照。M和BM处理池中总氨氮（TAN）含量（分别为0.03和0.01 mg l(-1)）和亚硝酸盐氮（NO(2)-N）含量（分别为0.03和0.01 mg l(-1)）显著低于B处理池(TAN 0.80、NO（2)-N 0.68 mg l(-1)）和对照池(TAN 1.11、NO（2)-N 1.12 mg l(-1)）。此外，与B和对照池相比，在M和BM池中培养的PL的存活率和特定生长率显著提高，对反盐胁迫试验的抗性也显著提高。与对照PL相比，BM池中培养的PL的蛋白质、脂肪、多不饱和脂肪酸、生态五烯酸和二十二碳六烯酸水平显著提高。BM池培养水中的弧菌含量也明显低于对照水。本研究结果说明了矮生双歧杆菌和周围生长微藻联合对改善孵化场水体质量和对虾生长和存活的有益作用。
We have investigated the possibility of using a consortium of marine bacterium and periphytic microalgae to improve the water quality and increase the growth and survival of the shrimp Penaeus monodon in a hatchery system. Three treatments were evaluated for their effect on P. monodon postlarvae (PL) when the culture water was not changed: Bacillus pumilus alone (B); periphytic microalgae alone (M); B. pumilus + periphytic microalgae (BM). P. monodon PL raised in a tank of unchanged water without bacterium and periphytic microalgae served as the control. The water in tanks of the M and BM treatments had significantly low levels of total ammonia-nitrogen (TAN) (0.03 and 0.01 mg l(-1), respectively) and nitrite-nitrogen (NO(2)-N) (0.03, 0.01 mg l(-1), respectively) than that in the B (TAN 0.80, NO(2)-N 0.68 mg l(-1)) and control (TAN 1.11, NO(2)-N 1.12 mg l(-1)) tanks. Moreover, PL cultured in tanks M and BM had significantly higher survival and specific growth rates and a significantly higher resistance to the reverse salinity stress test than those in the B and control tanks. Compared to the control PL, the PL cultured in the BM tanks had significantly higher levels of protein, lipid, polyunsaturated fatty acids, ecosapentaenoic acid, and docosahexaenoic acid. The culture water in tanks BM also contained significantly less Vibrio than the control water. Our results illustrate the beneficial effects of a B. pumilus and periphytic microalgae consortium on improving the water quality and the growth and survival of shrimp PL grown in a hatchery system.