Variable-constant segment genotype of immunoglobulin kappa is associated with increased risk for rheumatoid arthritis.
Variable-constant segment genotype of immunoglobulin kappa is associated with increased risk for rheumatoid arthritis.
复制标题
免疫球蛋白κ的可变恒定片段基因型与类风湿关节炎风险增加相关。
DOI：
10.1002/art.1780350104
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发表时间：
1992
影响因子：
--
通讯作者：
Moxley,G
中科院分区：
文献类型：
--
作者：
Moxley,G
目标。为了进一步研究类风湿关节炎（RA）与特定基因型的关系，该基因型由免疫球蛋白kappa （Ck）恒定段附近的限制性内切位点多态性鉴定。在Ck内或附近检测到的基因组DNA多态性（最Ck‐近端可变段[Vk] B3和T淋巴细胞标记物[CD8A]）的频率通过Southern印迹和杂交测定。结果：虽然B3、Km和CD8A基因型的频率在RA和正常对照人群中没有差异，但RA组中Ck和B3基因型的纯合子较多（相对危险度为2.2，P< 0.01），特别是DR4阴性RA亚组（相对危险度为3.9，P< 0.001）。包括ck片段在内的约30,000碱基区域的纯合基因型增加了RA的风险，特别是在DR4阴性亚组中。
Objective.To further investigate the association of rheumatoid arthritis (RA) with a particular genotype identified by a restriction site polymorphism near the constant segment of immunoglobulin kappa (Ck).Methods.The frequencies of genomic DNA polymorphisms detected within or near Ck(the most Ck‐proximal variable segment [Vk] B3 and a T lymphocyte marker [CD8A]) were determined by Southern blotting and hybridization. The frequencies of coding‐region polymorphisms of Ck(Km allotypes) were determined by amplification by polymerase chain reaction followed by restriction enzyme digestion.Results.Although the frequencies of B3, Km, and CD8A genotypes were not different between RA and normal control populations, more individuals were homozygous for both Ckand B3 in the RA group (relative risk 2.2,P< 0.01), especially in the DR4‐negative RA subgroup (relative risk 3.9,P< 0.001).Conclusion.The homozygous genotype of an approximately 30,000‐base region including the Cksegment confers an elevated risk for RA, particularly in the DR4‐negative subgroup.