Enzyme-Free and Label-Free Ultrasensitive Electrochemical Detection of Human Immunodeficiency Virus DNA in Biological Samples Based on Long-Range Self-Assembled DNA Nanostructures
Enzyme-Free and Label-Free Ultrasensitive Electrochemical Detection of Human Immunodeficiency Virus DNA in Biological Samples Based on Long-Range Self-Assembled DNA Nanostructures
复制标题
基于长距离自组装 DNA 纳米结构的生物样品中人类免疫缺陷病毒 DNA 的无酶无标记超灵敏电化学检测
DOI：
10.1021/ac3017828
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发表时间：
2012-10-02
影响因子：
7.4
通讯作者：
Yang, Huang-Hao
中科院分区：
文献类型：
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作者：
Chen, Xian;Hong, Cheng-Yi;Yang, Huang-Hao
基于纳米材料的生物传感器已被用于检测各种生物分子，具有高灵敏度和选择性。在此，我们开发了一个简单的和超灵敏的电化学DNA生物传感器使用长距离自组装DNA纳米结构作为载体的信号放大，它可以实现一个令人印象深刻的检测限S aM人类免疫缺陷病毒（HIV）DNA，即使在复杂的生物样品。在这项研究中，我们设计了两个辅助探头。两个辅助探针之间的级联杂交事件可以导致长距离自组装并形成微米长的一维DNA纳米结构。在靶DNA存在的情况下，靶的每个拷贝可以充当将DNA纳米结构连接到电极表面上的捕获探针的触发器。然后，大量的氧化还原指示剂[Ru（NH3）（6）]（3+）可以静电结合到DNA纳米结构上，并最终导致显着放大的电化学信号。
Biosensors based on nanomaterials have been used for detection of various biological molecules with high sensitivity and selectivity. Herein, we developed a simple and ultrasensitive electrochemical DNA biosensor using long-range self-assembled DNA nanostructures as carriers for signal amplification, which can achieve an impressive detection limit of S aM human immunodeficiency virus (HIV) DNA even in complex biological samples. In this study, we designed two auxiliary probes. A cascade of hybridization events between the two auxiliary probes can lead to long-range self-assembly and form micrometer-long one-dimensional DNA nanostructures. In the presence of target DNA, each copy of the target can act as a trigger to connect a DNA nanostructure to a capture probe on the electrode surface. Then, a great amount of redox indicator [Ru(NH3)(6)](3+) can be electrostatically bound to the DNA nanostructures and eventually result in significantly amplified electrochemical signals.