Simultaneous determination of multiclass illegal dyes with different acidic-basic properties in foodstuffs by LC-MS/MS via polarity switching mode
Simultaneous determination of multiclass illegal dyes with different acidic-basic properties in foodstuffs by LC-MS/MS via polarity switching mode
复制标题
采用极性切换模式的 LC-MS/MS 同时测定食品中不同酸碱性的多类非法染料
DOI：
10.1016/j.foodchem.2019.125745
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发表时间：
2020-03-20
期刊：
影响因子：
8.8
通讯作者：
Li, Chunyuan
中科院分区：
文献类型：
--
作者：
Hu, Zhibo;Qi, Ping;Li, Chunyuan
同时测定具有不同化学性质的多类非法染料是困难的。采用负离子/正离子切换的LC-MS/MS模式，建立了一种高效、快速、一次性检测食品中52种不同酸碱性质的非法染料的多残留方法，包括23种脂溶性中性偶氮染料、8种酸性磺化偶氮染料、12种三苯甲烷碱性染料、3种碱吲哚染料、3种杂蒽染料、1种喹啉染料和2种蒽醌染料。用甲醇-乙腈法提取非法染料，用d-SPE法进一步纯化以减少干扰。采用100倍稀释样品，消除LC-MS/MS分析定量时基质效应。验证结果表明，该方法回收率为71.2 ~ 111.2%，相对标准偏差小于20%，适用于食品中多类痕量非法染料的鉴别和定量。
Simultaneous determination of multiclass illegal dyes possessing different chemical properties is difficult. By using LC-MS/MS via negative/positive ion switching mode, an efficient and fast multi-residual method for simultaneous determination of multiclass 52 illegal dyes with different acidic-basic properties in foodstuffs was developed and validated during one single run, including 23 fat-soluble neutral azo dyes, 8 acidic sulfonated azo dyes, 12 triphenylmethane basic dyes, three basic indole dyes, three xanthene dyes, one quinoline dye, and two anthraquinones dyes. The illegal dyes were extracted with methanol-acetonitrile and further purified with d-SPE procedure to reduce interference. Sample dilution with 100-fold was used for the elimination of matrix effects of the quantitation of LC-MS/MS analysis. Validation data showed the good recoveries in the range of 71.2-111.2%, with relative standard deviations less than 20%, suggesting the developed method is suitable for the identification and quantitation of multiclass illegal dyes at trace levels in foods.