The XA21 binding protein XB25 is required for maintaining XA21-mediated disease resistance
The XA21 binding protein XB25 is required for maintaining XA21-mediated disease resistance
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DOI：
10.1111/tpj.12076
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发表时间：
2013-03-01
期刊：
影响因子：
7.2
通讯作者：
Song, Wen-Yuan
中科院分区：
文献类型：
--
作者：
Jiang, Yingnan;Chen, Xiuhua;Song, Wen-Yuan
植物基因组编码大量的蛋白质，这些蛋白质可能作为免疫受体在防御病原体入侵中发挥作用。水稻（Oryza sativa）蛋白XA 21是一种由23个富含亮氨酸的串联重复序列、一个跨膜结构域和一个丝氨酸/苏氨酸激酶组成的受体激酶，具有广谱的抗白叶枯病菌（Xanthomonaseruppv. nov.，Xoo）的特性。我们在这里报告，XA 21结合蛋白25（XB 25）属于植物特异性锚定重复序列（PANK）家族。在体外，XB 25通过其N端结合跨膜和带正电荷的结构域（BTMP）重复序列与XA 21的跨膜结构域发生物理相互作用。此外，在植物中，XB 25与XA 21结合。在成体阶段，Xb 25的下调导致XA 21水平降低和受损的XA 21介导的抗病性。此外，Xoo感染诱导了XB 25的积累。总之，这些结果表明，XB 25是维持XA 21介导的抗病性所必需的。
Plant genomes encode a large number of proteins that potentially function as immune receptors in the defense against pathogen invasion. As a well-characterized receptor kinase consisting of 23 tandem leucine-rich repeats, a transmembrane domain and a serine/threonine kinase, the rice (Oryza sativa) protein XA21 confers resistance to a broad spectrum of Xanthomonas oryzae pv.oryzae (Xoo) races that cause bacterial blight disease. We report here that XA21 binding protein 25 (XB25) belongs to the plant-specific ankyrin-repeat (PANK) family. XB25 physically interacts, in vitro, with the transmembrane domain of XA21 through its Nterminal binding to transmembrane and positively charged domain (BTMP) repeats. In addition, XB25 associates with XA21 in planta. The downregulation of Xb25 results in reduced levels of XA21 and compromised XA21-mediated disease resistance at the adult stage. Moreover, the accumulation of XB25 is induced by Xoo infection. Taken together, these results indicate that XB25 is required for maintaining XA21-mediated disease resistance.