Structural analysis and potential anti-tumor activity of Sporisorium reilianum (Fries) polysaccharide
Structural analysis and potential anti-tumor activity of Sporisorium reilianum (Fries) polysaccharide
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弗赖斯孢子多糖的结构分析及其潜在抗肿瘤活性
DOI：
10.1016/j.ijbiomac.2019.10.228
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发表时间：
2020-06-15
影响因子：
8.2
通讯作者：
Zhao, Min
中科院分区：
文献类型：
--
作者：
Kan, Lianbao;Chai, Yangyang;Zhao, Min
从玉米丝孢霉（Sporisorium reilianum（Fries））中分离纯化得到一种中性多糖WM-NP-60。研究了WM-NP-60的性质和潜在的抗肿瘤活性。WM-NP-60是一种水溶性多糖。WM-NP-60的分子量为15.6kDa。WM-NP-60的主链由β-1，6-D-Glcp组成，侧链由β-1，3-D-Glcp组成。侧链在C-3位与糖基结合到主链。Gal可以作为侧链连接到主链上或结合到线性β-1，3-D-Glcp侧链上。WM-NP-60对肝癌细胞HepG 2和胃癌细胞SGC 7901的增殖有抑制作用，且呈剂量依赖性。另外，WM-NP-60可使HepG 2和SGC 7901细胞周期阻滞于G（1）期，并诱导HepG 2和SGC 7901细胞凋亡。综上所述，这些结果表明，WM-NP-60具有抑瘤活性，可能被视为一种潜在的天然抗肿瘤药物。(C)2019爱思唯尔B. V.保留所有权利。
A neutral polysaccharide WM-NP-60 was successfully isolated and purified from a phytopathogenic fungus Sporisorium reilianum (Fries). The characteristics and potential antitumor activities of WM-NP-60 were studied. WM-NP-60 was a water-soluble polysaccharide. The molecular weight of WM-NP-60 was 15.6 kDa. The main chain of WM-NP-60 was composed of beta-1,6-D-Glcp and its side chains were beta-1,3-D-Glcp. The side chains bound to the main chain with glycosyl groups at the C-3 positions. Gal might be attached to the backbone as a side chain or bound to the linear beta-1,3-D-Glcp side chain. WM-NP-60 could inhibit the proliferation of HepG2 and SGC7901 cells in a dose-dependently manner. In addition, it was found that WM-NP-60 triggered the HepG2 and SGC7901 cell cycle arrest at the G(1) phase and induced apoptosis of HepG2 and SGC7901 cells. Taken together, these results suggested that WM-NP-60 possessed a tumor-suppressive activity and might be regarded as a potential natural anti-tumor drug. (C) 2019 Elsevier B.V. All rights reserved.