ZmWRKY79 positively regulates maize phytoalexin biosynthetic gene expression and is involved in stress response
ZmWRKY79 positively regulates maize phytoalexin biosynthetic gene expression and is involved in stress response
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ZmWRKY79正向调节玉米植物抗毒素生物合成基因表达并参与应激反应
DOI：
10.1093/jxb/erx436
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发表时间：
2018-01-23
影响因子：
6.9
通讯作者：
Wang, Qiang
中科院分区：
文献类型：
--
作者：
Fu, Jingye;Liu, Qin;Wang, Qiang
玉米（Zea mays）响应于各种激发而积累玉米萜类植物抗毒素（MTP）、贝壳杉素和玉米素。虽然这些关键的生物合成基因已被表征，但调控机制仍不清楚。通过相关性分析，转录因子（TF），ZmWRKY79，被确定为与MTP生物合成基因的表达高度相关。基因表达分析表明，ZmWRKY79是由禾谷镰刀菌感染，植物激素处理，和多重胁迫诱导。ZmWRKY79在玉米原生质体中的过表达增加了参与MTP生物合成、茉莉酸和乙烯途径以及活性氧（ROS）清除的基因的表达。随后在玉米原生质体中的瞬时RNAi破坏了茉莉酸和乙烯联合处理对MTP生物合成基因的诱导。这种调节进一步被证明是依赖于一个W盒或WLE顺式元件。ZmWRKY79在烟草中的瞬时过表达通过减少ROS产生而赋予对立枯丝核菌感染的抗性。我们的研究结果表明，MTP的生物合成是由共同的转录因子ZmWRKY79，它发挥了广泛的作用，作为一个潜在的主调节器，通过参与植物激素代谢或信号和ROS清除应激反应。
Maize (Zea mays) accumulates maize terpenoid phytoalexins (MTPs), kauralexins and zealexins in response to various elicitations. Although the key biosynthetic genes for these have been characterized, the regulatory mechanism remains unclear. Through co-correlation analysis, a transcription factor (TF), ZmWRKY79, was identified as highly correlated with expression of MTP biosynthetic genes. Gene expression analysis indicated that ZmWRKY79 was induced by Fusarium graminearum infection, phytohormone treatment, and multiple stresses. Overexpression of ZmWRKY79 in maize protoplasts increased expression of genes involved in MTP biosynthesis, jasmonic acid and ethylene pathways, and scavenging of reactive oxygen species (ROS). Subsequent transient RNAi in maize protoplast compromised the induction of MTP biosynthetic genes by jasmonic acid and ethylene combined treatment. Such regulation was further demonstrated to be dependent on a W-box or WLE cis-element. Transient overexpression of ZmWRKY79 in tobacco conferred resistance against Rhizoctonia solani infection through reducing ROS production. Our results indicate that MTP biosynthesis is regulated by the common transcription factor ZmWRKY79, which plays a broad role as a potential master regulator in stress response through involvement in phytohormone metabolism or signaling and ROS scavenging.