Simple and sensitive method for determination of glycoalkaloids in potato tubers by high-performance liquid chromatography with chemiluminescence detection
Simple and sensitive method for determination of glycoalkaloids in potato tubers by high-performance liquid chromatography with chemiluminescence detection
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DOI：
10.1016/j.chroma.2005.09.006
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发表时间：
2005-12-23
影响因子：
4.1
通讯作者：
Tanaka, Y
中科院分区：
文献类型：
--
作者：
Kodamatani, H;Saito, K;Tanaka, Y
基于三（2,2′-联吡啶）钌（III）的化学发光反应，建立了一种新型、简便、灵敏的高效液相色谱法测定马铃薯糖生物碱、-茄碱和-恰康碱的方法。在5 ng/ml-10 μ g/ml范围内，α -龙葵碱和（x-夏康碱）的校准图呈线性关系。-龙葵碱和-恰康碱的检出限分别为1.2和1.3 ng/ml。该方法成功地应用于马铃薯块茎样品，无需清洗，预浓缩和衍生化步骤。在10 μ g/g （n = 6）条件下，块茎髓中α -龙葵碱和x-查康碱的加标回收率分别为101.0 +/- 4.4%和103.6 +/- 7.1%，平均标准差为%。(c) 2005 Elsevier B.V.版权所有
A novel, simple and sensitive high-performance liquid chromatographic method for the determination of the potato glycoalkaloids, alpha-solanine and alpha-chaconine, based on the chemiluminescent reaction of tris(2,2'-bipyridine)ruthenium(III) has been developed. The calibration graph was linear in the range of 5 ng/ml-10 mu g/ml for both alpha-solanine and (x-chaconine. The detection limits of alpha-solanine and alpha-chaconine were 1.2 and 1.3 ng/ml, respectively. This method was successfully applied to a potato tuber sample without cleanup, pre-concentration, and derivatization steps. The recoveries (mean standard deviation, %) of alpha-solanine and (x-chaconine spiked in tuber pith at 10 mu g/g (n = 6) were 101.0 +/- 4.4% and 103.6 +/- 7.1%, respectively. (c) 2005 Elsevier B.V. All rights reserved.