A novel approach to regenerating periodontal tissue by grafting autologous cultured periosteum
A novel approach to regenerating periodontal tissue by grafting autologous cultured periosteum
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DOI：
10.1089/ten.2006.12.1227
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发表时间：
2006-05-01
影响因子：
--
通讯作者：
Ueda, Minoru
中科院分区：
文献类型：
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作者：
Mizuno, Hirokazu;Hata, Ken-Ichiro;Ueda, Minoru
在口腔颌面外科领域，组织工程技术已被发现可用于再生丢失的组织。牙周病会导致牙周组织（包括牙槽骨）严重破坏。在这项研究中，我们尝试通过移植来自骨膜的自体培养膜来再生犬牙周组织缺损。在适当的培养条件下，骨膜细胞产生足够的细胞外基质以形成片层。从成年杂交狗的下颌体上剥离骨膜标本并培养直至细胞形成膜。测量 ALP 活性以确定嫁接的最佳时间。将培养的骨膜 (CP) 移植并缝合在第 4 下颌前磨牙机械制造的 III 类分叉缺损上。 3个月后，收获样品并进行放射学和组织学观察。在 CP 病例中，骨缺损会再生并被新形成的硬组织填充，而在对照组中，骨缺损仍然存在。这些结果表明，我们的新疗法可有效再生牙槽骨以治疗牙周病。
In the field of oral and maxillofacial surgery, tissue-engineering techniques have been found useful in regenerating lost tissues. Periodontal disease causes severe destruction of periodontal tissue, including the alveolar bone. In this study we attempted to regenerate canine periodontal tissue defects by grafting autologous cultured membrane derived from the periosteum. Under appropriate culture conditions, periosteal cells produce enough extracellular matrix to form sheets. Periosteum specimens were peeled from the mandibular body of adult hybrid dogs and were cultured until cells formed membrane. ALP activity was measured to determine an optimal time for grafting. The cultured periosteum (CP) was grafted and sutured on a mechanically made Class III furcation defect in the 4(th) mandibular premolars. After 3 months, the samples were harvested and observed radiologically and histologically. In cases of CP, the bone defects were regenerated and filled with newly formed hard tissue, whereas in the controls the defects remained. These results show that our novel treatment is effective in regenerating alveolar bone for the treatment of periodontal disease.