Magnaporthe grisea infection triggers RNA variation and antisense transcript expression in rice
Magnaporthe grisea infection triggers RNA variation and antisense transcript expression in rice
复制标题
DOI：
10.1104/pp.107.095653
复制
发表时间：
2007-05-01
期刊：
影响因子：
7.4
通讯作者：
Wang, Guo-Liang
中科院分区：
文献类型：
--
作者：
Gowda, Malali;Venu, R.-C.;Wang, Guo-Liang
由稻瘟病菌引起的稻瘟病是研究植物-真菌互作和寄主防御反应的理想模式系统。本研究综合分析了M.使用基因表达的稳健长序列分析进行稻瘟病菌感染。从分离自抗性（R）、敏感（S）和对照（C）文库的627，262个单独的标签中鉴定了总共83，382个不同的21-bp稳健长序列分析的基因表达标签。序列分析表明，与C文库相比，R和S文库中的标签与水稻基因组和表达序列的匹配率显著降低。R和S文库标签的高水平的一个核苷酸错配是由于核苷酸转换。A → G和U → C的核苷酸转换是最主要的类型，在M.受稻瘟病菌感染的植物逆转录-聚合酶链反应分析表明，腺嘌呤脱氨酶和胞苷脱氨酶基因的表达高度诱导接种后。此外，在感染的植株中诱导了许多反义转录物，并且通过链特异性逆转录-聚合酶链反应证实了四种反义转录物的表达。这些结果表明，水稻转录组在M.稻瘟病菌感染早期阶段。
Rice blast disease, caused by the fungal pathogen Magnaporthe grisea, is an excellent model system to study plant-fungal interactions and host defense responses. In this study, comprehensive analysis of the rice (Oryza sativa) transcriptome after M. grisea infection was conducted using robust-long serial analysis of gene expression. A total of 83,382 distinct 21-bp robust-long serial analysis of gene expression tags were identified from 627,262 individual tags isolated from the resistant (R), susceptible (S), and control (C) libraries. Sequence analysis revealed that the tags in the R and S libraries had a significant reduced matching rate to the rice genomic and expressed sequences in comparison to the C library. The high level of one-nucleotide mismatches of the R and S library tags was due to nucleotide conversions. The A-to-G and U-to-C nucleotide conversions were the most predominant types, which were induced in the M. grisea-infected plants. Reverse transcription-polymerase chain reaction analysis showed that expression of the adenine deaminase and cytidine deaminase genes was highly induced after inoculation. In addition, many antisense transcripts were induced in infected plants and expression of four antisense transcripts was confirmed by strand- specific reverse transcription- polymerase chain reaction. These results demonstrate that there is a series of dynamic and complex transcript modifications and changes in the rice transcriptome at the M. grisea early infection stages.