During in vivo maturation of eukaryotic nuclear mRNA, splicing yields excised exon circles
During in vivo maturation of eukaryotic nuclear mRNA, splicing yields excised exon circles
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DOI：
10.1093/nar/24.6.1015
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发表时间：
1996-03-15
影响因子：
14.9
通讯作者：
Bailleul, B
中科院分区：
文献类型：
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作者：
Bailleul, B
在ETS-1基因的多外显子和Sry基因的单个1.2 kb外显子中，核前mrna上的某些剪接位点已经被描述为圆形剪接，迄今为止，尚不清楚剪接位点并并如何发生在正常剪接和圆形剪接中，内部外显子的剪接位点选择可能涉及到该外显子上剪接位点之间的配对，基于此，我们预测，尽管频率较低，内部外显子通过剪接产生环状RNA，这是一种易出错的外显子并置机制，或者更有趣的是，作为一种调节外显子的机制，为了解决这个问题，我们分析了人类细胞系和血细胞样本中三个ETS-1内部外显子（一个选择剪接外显子和两个组成外显子）的环状外显子形成。在这里，我们通过RT-PCR和测序显示，外显子环状剪接发生在我们检查的三个单独的外显子上。RNase保护实验表明外显子环表达与外显子跳变之间没有相关性。
Circular splicing has already been described on nuclear pre-mRNA for certain splice sites far apart in the multi exonic ETS-1 gene and in the single 1.2 kb exon of the Sry locus, To date, it is unclear how splice site juxtaposition occurs in normal and circular splicing, The splice site selection of an internal exon is likely to involve pairing between splice sites across that exon, Based on this, we predict that, albeit at low frequency, internal exons yield circular RNA by splicing as an error-prone mechanism of exon juxtaposition or, perhaps more interestingly, as a regulated mechanism on alternative exons, To address this question, the circular exon formation was analysed at three ETS-1 internal exons (one alternative spliced exon and two constitutive), in human cell line and blood cell samples, Here, we show by RT-PCR and sequencing that exon circular splicing occurs at the three individual exons that we examined, RNase protection experiments suggest that there is no correlation between exon circle expression and exon skipping.