Sexually dimorphic expression of foxl2 during gametogenesis in scallop Chlamys farreri, conserved with vertebrates
Sexually dimorphic expression of foxl2 during gametogenesis in scallop Chlamys farreri, conserved with vertebrates
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扇贝栉孔扇贝配子发生过程中foxl2的性别二态性表达，在脊椎动物中保守
DOI：
10.1007/s00427-012-0410-z
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发表时间：
2012-09-01
影响因子：
2.4
通讯作者：
Muhammad, Faiz
中科院分区：
文献类型：
--
作者：
Liu, Xiao-Ling;Zhang, Zhi-Feng;Muhammad, Faiz
FOXL2是叉头盒家族的成员之一，它是一种转录因子，在脊椎动物中以性别二态的方式表达，对卵巢发育的早期调节至关重要。然而，关于无脊椎动物这种基因的数据很少。在本研究中，我们克隆了栉孔扇贝FOXL2基因的全长序列。栉孔扇贝是一种重要的商业贝类，属于中国。Cf-FOXL2(C.farreri FOXL2)的全长为1,824个碱基，开放阅读框为1,107个碱基，编码369个氨基酸残基，含保守结构域叉头盒。半定量RT-PCR结果表明，CF-FOXL2主要在卵巢中表达。利用实时荧光定量聚合酶链式反应发现，增殖期动物卵巢中的表达水平最高，约为睾丸的62倍，约为生长期和成熟期的2倍。原位杂交结果显示，CFFOXL2mRNA定位于卵泡细胞和生殖细胞的胞浆中，在卵巢和睾丸中，除精子外，其他部位均有表达。我们的数据表明，Cf-FOXL2在RNA水平上以性别二态的模式表达，这在脊椎动物中是保守的。
FOXL2 is a member of the forkhead box family, a transcription factor essential for the early regulation of ovarian development that is expressed in a sexually dimorphic manner in vertebrates. However, data on this gene in invertebrates are rare. In this study, we cloned a full-length cDNA sequence of foxl2 from the scallop Chlamys farreri, an important commercial mollusk in China. The cDNA sequence of Cf-foxl2 (C. farreri foxl2) has 1,824 bp with an open reading frame of 1,107 bp encoding 369 amino acid residues containing the conserved domain forkhead box. Semiquantitative RT-PCR showed that Cf-foxl2 was expressed mainly in the ovary. Using quantitative real-time PCR, we found that the highest expression was in the ovary of proliferative stage animals, about 62-fold higher than that in the testis and about twofold higher than that in the ovary of growing and mature stages. In situ hybridization revealed that Cf-foxl2 mRNA was located in the cytoplasm of follicle cells and germ cells in the ovary and testis except in the spermatozoa. Our data imply that Cf-foxl2 is expressed in a sexually dimorphic pattern at the RNA level, which is conserved with vertebrates.