Expression analysis of two Eomesodermin homologues in zebrafish lymphoid tissues and cells.
Expression analysis of two Eomesodermin homologues in zebrafish lymphoid tissues and cells.
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DOI：
10.1016/j.molimm.2006.11.018
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发表时间：
2007-03
影响因子：
3.6
通讯作者：
Fumio Takizawa;Kyosuke Araki;Kanako Ito;T. Moritomo;T. Nakanishi
中科院分区：
文献类型：
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作者：
Fumio Takizawa;Kyosuke Araki;Kanako Ito;T. Moritomo;T. Nakanishi
Eomesodermin（Eomes）是一种T盒转录因子，参与大多数脊椎动物中胚层的形成。Eomes也在CD 8 +T细胞和NK细胞中表达。迄今为止，没有关于Eomes在低等脊椎动物免疫系统中作用的信息，尽管已经在斑马鱼中进行了Eomes（Eomes 1）的发育研究。在这里，我们报告的第二个Eomes（Eomes 2）在斑马鱼的识别和比较两个Eomes基因在免疫系统中的表达。斑马鱼Eomes 1和Eomes 2分别由661和534个氨基酸组成，编码区氨基酸同源性为49.3%，T-box区氨基酸同源性为88.7%。人类和斑马鱼基因组区域之间的保守同线性，基因组织和系统发育分析都表明，斑马鱼Eomes 2基因是哺乳动物Eomes的同源物，如先前发现的斑马鱼Eomes 1。Eomes 1 mRNA在性腺、体肾、脾脏和鳃中均有表达，而Eomes 2 mRNA在这些组织中均未检测到。然而，两种Eomes mRNA的强表达在来自脾的白细胞中检测到，其次是来自体肾和外周血的白细胞，Eomes 1的表达总是强于Eomes 2的表达。通过流式细胞仪分选的体肾细胞的RT-PCR分析显示，Eomes 1在淋巴细胞中强表达，在母细胞中弱表达，并且在粒细胞中不表达，而Eomes 2在淋巴细胞中弱表达。这些结果表明，这两个Eomes基因参与斑马鱼的免疫反应，特别是在淋巴细胞功能，已经发现在哺乳动物。
Eomesodermin (Eomes) is a T-box transcription factor that is involved in mesoderm formation in most vertebrates. Eomes is also expressed in CD8+T cells and NK cells. No information is available on the role of Eomes in the immune system of lower vertebrates to date, although developmental studies on Eomes (Eomes1) have been performed in zebrafish. Here we report the identification of a second Eomes (Eomes2) in zebrafish and compare expression of the two Eomes genes in the immune system. Zebrafish Eomes1 and Eomes2, composed of 661 and 534 amino acids, respectively, share 49.3% amino acid identity in their coding regions and 88.7% amino acid identity in their T-box regions. Conserved synteny between regions of the human and zebrafish genomes, gene organization and phylogenetic analysis all indicate that the zebrafish Eomes2 gene is a homologue of mammalian Eomes, as previously found for zebrafish Eomes1. Eomes1 mRNA was found to be expressed in the gonad, body kidney, spleen and gill, while Eomes2 mRNA was not detected in any of these tissues. However, strong expression of both Eomes mRNAs was detected in the leukocytes from the spleen, followed by those from body kidney and peripheral blood, with expression of Eomes1 always stronger than that of Eomes2. RT-PCR analysis of body kidney cells sorted by FACS revealed that Eomes1 was expressed strongly in lymphocytes, weakly in blast cells, and was not expressed in granulocytes, while Eomes2 was expressed weakly in lymphocytes. These results suggest that both Eomes genes are involved in the zebrafish immune response, particularly in lymphocyte function as has been found in mammals.