ESTABLISHMENT AND CHARACTERIZATION OF A MONOCLONAL-ANTIBODY RECOGNIZING THE DEWAR ISOMERS OF (6-4)PHOTOPRODUCTS
ESTABLISHMENT AND CHARACTERIZATION OF A MONOCLONAL-ANTIBODY RECOGNIZING THE DEWAR ISOMERS OF (6-4)PHOTOPRODUCTS
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DOI：
10.1111/j.1751-1097.1993.tb02952.x
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发表时间：
1993-06-01
影响因子：
3.3
通讯作者：
NIKAIDO, O
中科院分区：
文献类型：
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作者：
MATSUNAGA, T;HATAKEYAMA, Y;NIKAIDO, O
我们建立了一种能识别除环丁烷、嘧啶二聚体或(6-4)光产物以外的紫外线诱导的DNA损伤的单抗(DEM-1)。DEM-1抗体与254 nm紫外光照射DNA的结合力随紫外光照射波长大于310 nm而增加，而针对(6-4)光产物的64M-2抗体的结合率随处理时间的延长而降低。此外，在暴露期间，64M-2抗体的存在抑制了DEM-1结合的增加。我们得出结论，DEM-1抗体特异性地识别杜瓦光产物，它是(6-4)光产物的异构体。然而，DEM-1抗体也与254 nm紫外光照射的DNA结合，这表明254 nm紫外光可以诱导杜瓦光产物，而不会随后暴露于更长波长的紫外光。此外，作用光谱研究表明，254 nm是杜瓦光产物诱导的最有效波长，在254-365 nm区域，以及环丁烷二聚体和(6-4)光产物，尽管在UV-B区的作用谱值明显高于环丁烷二聚体和(6-4)光产物诱导。
We established a monoclonal antibody (DEM-1) that recognizes UV-induced DNA damage other than cyclobutane pyrimidine dimers or (6-4)photoproducts. The binding of DEM-1 antibody to 254 nm UV-irradiated DNA increased with subsequent exposure to UV wavelengths longer than 310 nm, whereas that of the 64M-2 antibody specific for the (6-4)photoproduct decreased with this treatment. Furthermore, the increase in DEM-1 binding was inhibited by the presence of the 64M-2 antibody during the exposure. We concluded that the DEM-1 antibody specifically recognized the Dewar photoproduct, which is the isomeric form of the (6-4)photoproduct. The DEM-1 antibody, however, also bound to DNA irradiated with high fluences of 254 nm UV, suggesting that 254 nm UV could induce Dewar photoproducts without subsequent exposure to longer wavelengths of UV. Furthermore, an action spectral study demonstrated that 254 nm was the most efficient wavelength for Dewar photoproduct induction in the region from 254 to 365 nm, as well as cyclobutane dimers and (6-4)photoproducts, although the action spectrum values in the UV-B region were significantly higher compared with those for cyclobutane dimer and (6-4)photoproduct induction.