The p24 proteins are not essential for vesicular transport in Saccharomyces cerevisiae
The p24 proteins are not essential for vesicular transport in Saccharomyces cerevisiae
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DOI：
10.1073/pnas.070044097
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发表时间：
2000-04-11
影响因子：
11.1
通讯作者：
Schekman, R
中科院分区：
文献类型：
--
作者：
Springer, S;Chen, E;Schekman, R
为了研究货物蛋白分选到COPII内质网(ER)到高尔基体运输小泡的因素，我们创造了一株酿酒酵母(P24 Delta 8)，它缺乏p24家族的所有八个跨膜蛋白成员(Emp24p、Erv25p和Erp1p到Erp6p)。P24蛋白参与了真核细胞中COPI和COPII囊泡的形成、货物蛋白的分选和囊泡运输的调节。我们发现p24 Delta 8细胞的生长与野生型相同，转化酶和Gas1p ER到高尔基体运输的延迟与单个Delta emp24缺失菌株中的相同。因此，p24蛋白在分泌途径中不具有必要的功能。相反，它们可能成为限制蛋白质进入COPII囊泡的质量控制因素。
To investigate the factors involved in the sorting of cargo proteins into COPII endoplasmic reticulum (ER) to Golgi apparatus transport vesicles, we have created a strain of S. cerevisiae (p24 Delta 8) that lacks all eight members of the p24 family of transmembrane proteins (Emp24p, Erv25p, and Erp1p to Erp6p). The p24 proteins have been implicated in COPI and COPII vesicle formation, cargo protein sorting, and regulation of vesicular transport in eukaryotic cells. We find that p24 Delta 8 cells grow identically to wild type and show delays of invertase and Gas1p ER-to-Golgi transport identical to those seen in a single Delta emp24 deletion strain. Thus, p24 proteins do not have an essential function in the secretory pathway. instead, they may serve as quality control factors to restrict the entry of proteins into COPII vesicles.