Specific point mutations may not accumulate with aging in the mouse mitochondrial DNA control region.
Specific point mutations may not accumulate with aging in the mouse mitochondrial DNA control region.
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DOI：
10.1016/j.gene.2005.02.008
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发表时间：
2005-05
期刊：
影响因子：
3.5
通讯作者：
Xiufeng Song;Jian‐Hong Deng;C. J. Liu;Yidong Bai
中科院分区：
文献类型：
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作者：
Xiufeng Song;Jian‐Hong Deng;C. J. Liu;Yidong Bai
越来越多的证据表明，在各种组织和广泛的生物体中，线粒体功能在衰老过程中下降。这与最近在人类成纤维细胞和骨骼肌中报道的mtDNA控制区中特定点突变的年龄依赖性大量积累有关。然而，在其他模型动物系统中评估与衰老相关的mtDNA突变。在这项研究中，我们分析了老年小鼠脑，骨骼肌，心脏和其他组织的mtDNA控制区，以寻找特定的点突变。对25-26月龄小鼠不同组织中mtDNA控制区的948 bp片段进行了测序。用新开发的程序Mutation Quantifier完成序列分析，该程序能够准确检测频率低至3%的突变。可能是由于小鼠寿命相对较短，与人类mtDNA不同，我们的研究结果表明，在衰老过程中，小鼠mtDNA控制区可能没有明显的特定突变积累。
Increasing evidence suggests that mitochondrial function declines during aging in various tissues and in a wide range of organisms. This correlates with an age-dependent large accumulation of specific point mutations in the mtDNA control region that was reported recently in human fibroblast and skeletal muscle. However, evaluations of aging-related mtDNA mutations in other model animal systems. In this study, we analyzed mtDNA control regions of brain, skeletal muscle, heart, and other tissues from aged mice, in search of specific point mutations. A 948-bp fragment covering the entire mtDNA control region from various tissues of mice at the age of 25–26 months was sequenced. The sequence analysis was accomplished with a newly developed program Mutation Quantifier, which was able to accurately detect mutations with frequencies as low as 3%. Probably due to the relative shorter life-span, unlike what has been reported in human mtDNA, our results indicated there might be no significant accumulation of specific mutations in mouse mtDNA control region during aging.